ICC/IHC/Flow Markers Flashcards
1
Q
Actin sarcomeric
A
striated muscle
2
Q
Actin smooth muscle
A
smooth muscle
3
Q
amylin (IAPP)
A
pancreas (islet amyloid)
4
Q
CD1a
A
cortical thymocytes, langerhans cells, T cell lymphoblastic, dendritic cells, feline progressive systemic histiocytosis, reactive/systemic histiocytosis
5
Q
CD1c
A
dendritic cells, feline progressive histiocytosis
6
Q
CD3
A
T cell
7
Q
CD4
A
T cell (helper), neut, mac
8
Q
CD5
A
T cell
9
Q
CD8a
A
T cell
10
Q
CD8b
A
T cell
11
Q
CD10
A
Renal, stromal tumors
12
Q
CD11b
A
granulocytes, monocytes/macs
13
Q
CD11c
A
granulocytes, monocytes, dendritic cells
14
Q
CD11d
A
lymphoid, histiocytic tumors
15
Q
CD14
A
monocytes/macs
16
Q
CD18
A
leukocytic tumors (mono/mac, granulocyte)
17
Q
CD20
A
B cell
18
Q
CD21
A
B cell
19
Q
CD31
A
vascular endothelial, megakaryocytic tumors, monos
20
Q
CD34
A
HSC, vascular neoplasms
21
Q
CD41/61
A
megakaryocytes, PLTs
22
Q
CD45
A
leukocytic tumors
23
Q
CD45RA
A
lymphoid tumors
24
Q
CD68
A
macs, myeloid leukemia
25
CD71
reticulocytes
26
CD79a
B cell
27
CD90
interstitial dendritic cells, reactive/systemic histiocytosis
28
CD117
MCT, GIST, melanoma
29
CD163
histiocytic (phagocytic) sarcoma
30
CD204
histiocytic (phagocytic) sarcoma
31
Calcitonin
C cell (medullary) thyroid tumor
32
Calponin
smooth muscle, myofibroblastic + myoepi tumors
33
Calprotectin
macs, myeloid cells
34
calretinin
renal tubules, nerve tissue, adrenocortical tumors, mesothelioma
35
chromogranin A
neuroendocrine
36
caludin 1
epithelial tumors, meningioma
37
CNPase
PNST (Schwann cells), oligodendroglioma
38
COX–1
normal urothelium, endothelium
39
cytokeratin 5
myoepithelium, epi basal cells, mesothelium
40
cytokeratin 7
glandular epi neoplasms
41
cytokeratin 8/18
glandular epi neoplasms
42
cytokeratins AE1–AE3
general epi cell marker
43
cytokeratins PAN
general epi cell marker
44
cytokeratins HMW
squamous epithelium, mesothelium
45
desmin
muscle tumors
46
E–cadherin
landerhans cells, epi neoplasms, canine histiocytoma, meningioma
47
F.VIII–related Ag
vascular endothelial, megakaryocytic tumors
48
GATA–4
sex cord stromal tumors
49
GFAP
neural (glial) tumors, astrocytoma
50
glut 1
peripheral nerves, stromal cells, kidney
51
glycophorin A
erythroid leukemia
52
inhibin–alpha
sex cord stromal, adrenocortical tumors
53
Ki–67
cell proliferation markers
54
laminin
PVWT, basement membrane
55
lysozyme
histiocytes (macs)
56
LYVE–1
lymphatic endothelial tumors
57
melan–A
melanocytic neoplasms, steroid–producing tumors
58
MHCII
mac, dendritic lineage, APCs, lymphs
59
microphthalmia transcription factor
melanocytic neoplasms
60
MPO
granulocytic leukemia
61
MyoD1
rhabdomyosarcoma
62
myoglobin
skeletal muscle tumors
63
myosin
smooth muscle tumors
64
NSE
neuroendocrine
65
OCT–3/4
germ cells, stem cells
66
Olig–2
oligodendroglioma
67
p63
myoepithelium, epi basal cells, urothelial tumors
68
pax5
B cell
69
PCNA
proliferation marker
70
protein gene product 9.5
neuroendocrine
71
prox–1
lymphatic endothelial tumors
72
S100
neural marker, neuroendocrine tumors
73
somatostatin
pancreatic islet tumor, some carcinoids
74
synaptophysin
neuroendocrine
75
tryptase
MCT
76
tyrosinase
melanocytic tumors
77
uroplakin III
urothelial tumors
78
vimentin
mesenchymal tumors
79
Adrenal cortex
melan–A, inhibin–alpha, calretinin
80
adrenal medulla
PGP 9.5, chromogranin, synaptophysin
81
endocrine (generic)
chromogranin A, synaptophysin, PGP 9.5, NSE, S100
82
epithelial (vs mesenchymal)
cytokeratins, E–cadherin, claudin–1, p63
83
mesenchymal (vs epithelial)
vimentin, p63
84
hepatocytes
hep par 1
85
biliary epithelium
cytokeratin 7, 19
86
all muscle
actin muscle, desmin
87
skeletal muscle
actin sarcomeric, myoglobin
88
smooth muscle
calponin, SMA
89
SCC
CK5, p63
90
adenocarcinoma
CK7, CK8/18
91
sex–cord stromal tumor
inhibin–alpha, NSE
92
germ cell tumor
calretinin, KIT, Oct3/4, PGP 9.5
93
thyroid follicular cell
thyroglobulin
94
thyroid medulla, C cell
calcitonin
95
endothelial tumors
F.VIIIrp, CD31, CD34 (blood and lymphatic); LYVE–1, Prox–1 (lymphatic)
96
histiocytic sarcoma
CD18+, CD11d–
97
hemophagocytic sarcoma
CD18+, CD11d+
98
cutaneous histiocytoma
e–cadherin+, CD45+, vimentin+
99
alcian blue
sialomucins, hyaluronic acid, sulfated mucosubstances
100
alizarin red S
calcium
101
best carmine
glycogen
102
bielschowsky silver stain
axons
103
congo red
amyloid
104
cresyl violet
nissl substance
105
dunn–thompson
Hb
106
feulgen
DNA
107
fontana–masson
melanin
108
gordon and sweets reticulin fiber
reticulin fibers
109
grimelius
argyrophilic granules
110
hall
bile/biliverdin
111
jone methenamine
basement membrane
112
kinyoun (mod Ziehl–neelsen)
lipofuscin
113
luxol fast blue
myelin
114
mallory PTAH
muscle, fibrin, glial processes
115
masson trichrome
muscle, collagen
116
mayer mucicarmine
mucin, hyaluronic acid, chondroitin sulfate
117
methyl green pyronin
nucleic acids
118
oil red o
fat
119
PAS
glycogen, mucin
120
prussian blue
iron
121
rhodanine / rubeanic acid
copper
122
schmorl reaction
melanin, lipofuscin
123
toluidine blue
mast cells
124
verhoeff
elastic fibers
125
von kossa
calcium
126
jimenez, macchiavello
chlamydiae
127
wade–fite, ziehl–neelsen
acid fast bacteria
128
CD16 / CD56
NK cell
129
LMW cytokeratins
CK 7,8,18
130
HMW cytokeratins
CK 1–6,9,10,14
131
osteoblasts
ACP, ALP, osteocalcin
132
osteoclasts
TRAP, RANK, MHCII
133
hibernoma
UCP–1
134
sudan black B, osmium
lipid
