IC5 Flashcards
What cells produce MHC class I?
nucleated cells and platelets
What cells produce MHC class II?
APCs, B cells
What types of antigen does MHC class I bind to?
endogeneous: own, virus, neoantigen
What types of antigen does MHC class II bind to?
exogenous: bacteria
Which MHC class binds to CD8+
class I
Explain the process of antigen presentation by MHC I
endogeneous protein already in cytoplasm is broken down into peptides by proteasome.
Explain the process of antigen presentation by MHC II
exogeneous protein is phagocytosed into cell
How have T cells adapted to produce intracellular signals despite a short cytoplasmic tail?
CD3 proteins form dimers- CDey, CDEs, CDzz that contain ITAMs. ITAMs contain tyr which are phosphorylated to initiate signal transduction
How many CDRs are in T cells and antibodies
6 and 12
How, when and where does hypervariability of CDRs occurs in B cells?
In bone marrow, naive B cells undergo genetic rearrangement of variable domain ->clones are different
How, when and where does hypervariability of CDRs occurs in T cells?
Naive T cells in thymus undergo genetic recombination in variable domains. Va undergoes VJ recombination while VB undergoes DJ and then VDJ recombination
Whats the difference between monoclonal and polyclonal antibodies?
Monoclonal ab produced from the same B cell clone and only recognises one epitope.
polyclonal ab is a mixture of monoclonal ab
How are polyclonal antibodies made?
from passive immunisation of animals and collecting their antiserum
What 2 ways is antiserum purified? Which purification method results in the isolation of more antigen-specific antibodies
proteins A/G and
Immunoaffinity column chromatography. The latter
What modification is done in chimeric mabs?
all seq humanised except V domains
