I hate This Class 1 🧫🦠Flashcards
What determines the resolution of light microscopy
It is determined by the wavelength
The minimum distance to see two separate things (will show resolution)
Which microscopy allows you to see proteins on edges? What about edges of the cell?
What is the difference between an antigen and an epitope
How do you make a monoclonal antibody polyclonal?
Draw an antibody and label its parts
How are proteins sorted on native gels? SDS-Page gels? B mercaptoethanol gels? What if the reduction is not complete?
Explain CoIP
(CoIminoPrecipitation)
Explain the three types of chromatography
Which chromatography and gel techniques can be used to study diners
Explain forward vs reverse genetics
Describe what it means for a membrane to be energetically favorable
What factors influence membrane rigidity
If a lipid has a longer or shorter tail how does that effect it’s interactions with other lipids
Are lipids different across bilayers ? Between Organelles?
Yes
Hydropathy plots explain
Describe different ways protein mobility is restricted
Draw Frap experiment based off of protein mobility
What causes the initial delay in microfilament growth
What does cofilin do? Tropomyosin?
What does formin do? Who does formin bind?
What does Capz do? Tropomodulin? Arp2/3?
What does thymosin do? Profilin?
What would a sample of actin look like with different signal proteins look like ie(tropomyosin) what if you took them away?
Describe basic structure of microtubules Draw monomers and whole tubules. How do they add? Where is the GTP?
What accessory proteins help with nucleation of microtubules
Describe higher order microtubule structures (centrioles, cilia, centrosomes, MTOCs) how many rings do they have? Doublets triplets? Know the numbers
Describe microtubule accessory proteins. (Maps, kinesin-13, XMAP215, Y-TuRC, y-TuSC
How do intermediate filaments provide stretching force to the cell
Where and when can Intermediate filament subunits be replaced
How can the knowledge of localized intermediate filaments to target specific organs? How is this Information used in cancer diagnosis?
Which molecular motors travel on actin?
On microtubules?
Which direction do dynein or kinesin travel? Which direction do different myosins travel (1,2,5,6)? Which motors are processive? Which are dimers? Which are used for transport?
Myosin movement with ATP/ADP
Kinesin? Dynein?
Where does cargo bind to the different motor proteins
Describe how motors attached to a fluorescente filament where will the protein travel (this depends on motor)
Which direction would dynein travel in an epithelial cell/ or neuron
Draw a picture of cells sorting themselves based off of principles of homologous Cadherin binding and high and low concentration binding
Hemidesmosomes : focal adhesions: desmosomes : adherens junctions:
Which use cadherens which use inigrins? Which connect to actin filaments? Which connect to intermediate filaments? Which junctions use catenins And viculins? Which use talin and vinculin? Which use plectin adapters? What would happen if you got rid of said things?
How are integrins activated? How about cadherins?
Describe tight junctions how do they prevent passage across cell barriers?
What channels allow passage of small molecules between cells
Describe RNA maturation and ribosome production. What happens to the 45 s RNA? What do snoRNPs do? What happens to RNA bases?
Describe the nuclear envelope? What organelle is it continuous with? What cytoskeletal protiens line the inside? What about the outside? How is it held there? Describe the linc complex
Where are transcription factors? Heterochromatin vs euchrmatin?
