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Question 1

What determines the resolution of light microscopy

Answer 1

It is determined by the wavelength

The minimum distance to see two separate things (will show resolution)

Question 2

Which microscopy allows you to see proteins on edges? What about edges of the cell?

Question 3

What is the difference between an antigen and an epitope

Question 4

How do you make a monoclonal antibody polyclonal?

Question 5

Draw an antibody and label its parts

Question 6

How are proteins sorted on native gels? SDS-Page gels? B mercaptoethanol gels? What if the reduction is not complete?

Question 7

Explain CoIP
(CoIminoPrecipitation)

Question 8

Explain the three types of chromatography

Question 9

Which chromatography and gel techniques can be used to study diners

Question 10

Explain forward vs reverse genetics

Question 11

Describe what it means for a membrane to be energetically favorable

Question 12

What factors influence membrane rigidity

Question 13

If a lipid has a longer or shorter tail how does that effect it’s interactions with other lipids

Question 14

Are lipids different across bilayers ? Between Organelles?

Answer 14

Yes

Question 15

Hydropathy plots explain

Question 16

Describe different ways protein mobility is restricted

Question 17

Draw Frap experiment based off of protein mobility

Question 18

What causes the initial delay in microfilament growth

Question 19

What does cofilin do? Tropomyosin?

Question 20

What does formin do? Who does formin bind?

Question 21

What does Capz do? Tropomodulin? Arp2/3?

Question 22

What does thymosin do? Profilin?

Question 23

What would a sample of actin look like with different signal proteins look like ie(tropomyosin) what if you took them away?

Question 24

Describe basic structure of microtubules Draw monomers and whole tubules. How do they add? Where is the GTP?

Question 25

What accessory proteins help with nucleation of microtubules

Question 26

Describe higher order microtubule structures (centrioles, cilia, centrosomes, MTOCs) how many rings do they have? Doublets triplets? Know the numbers

Question 27

Describe microtubule accessory proteins. (Maps, kinesin-13, XMAP215, Y-TuRC, y-TuSC

Question 28

How do intermediate filaments provide stretching force to the cell

Question 29

Where and when can Intermediate filament subunits be replaced

Question 30

How can the knowledge of localized intermediate filaments to target specific organs? How is this Information used in cancer diagnosis?

Question 31

Which molecular motors travel on actin?
On microtubules?

Question 32

Which direction do dynein or kinesin travel? Which direction do different myosins travel (1,2,5,6)? Which motors are processive? Which are dimers? Which are used for transport?

Question 33

Myosin movement with ATP/ADP
Kinesin? Dynein?

Question 34

Where does cargo bind to the different motor proteins

Question 35

Describe how motors attached to a fluorescente filament where will the protein travel (this depends on motor)

Question 36

Which direction would dynein travel in an epithelial cell/ or neuron

Question 37

Draw a picture of cells sorting themselves based off of principles of homologous Cadherin binding and high and low concentration binding

Question 38

Hemidesmosomes : focal adhesions: desmosomes : adherens junctions:

Which use cadherens which use inigrins? Which connect to actin filaments? Which connect to intermediate filaments? Which junctions use catenins And viculins? Which use talin and vinculin? Which use plectin adapters? What would happen if you got rid of said things?

Question 39

How are integrins activated? How about cadherins?

Question 40

Describe tight junctions how do they prevent passage across cell barriers?

Question 41

What channels allow passage of small molecules between cells

Question 42

Describe RNA maturation and ribosome production. What happens to the 45 s RNA? What do snoRNPs do? What happens to RNA bases?

Question 43

Describe the nuclear envelope? What organelle is it continuous with? What cytoskeletal protiens line the inside? What about the outside? How is it held there? Describe the linc complex

Question 44

Where are transcription factors? Heterochromatin vs euchrmatin?