Hybridization Intro Flashcards
1
Q
3 types of hybridization?
A
solution, solid, in-situ
2
Q
Applications of hybridization
A
gg
3
Q
via which denaturation occurs
A
gg
4
Q
Are mismatches tolerated in hybridization?
A
yes
5
Q
What happens when mismatches are present? Draw a mismatched duplex
A
weak
6
Q
Degree of specificity of binding of 2 chains is affected by? how each affect?
A
T, pH, [Salt], destab.
7
Q
What is stringency? Types with conditions?
A
The strictness of conditions which determines the degree of mismatch that can be tolerated to maintain duplex structure
8
Q
Specificity of a probe depends on what?
A
condi
9
Q
What is the objective in changing conditions?
A
allow
minimize
10
Q
What is generally being done?
A
low string.
post-washes
11
Q
What are the 4 components in a hybridization reaction?
A
target
probe
label
hybri. format
