HUNT FOR GENETIC MATERIAL Flashcards
transforming principle was given by
Fredrick Griffith
transforming principle was conducted on
mice and streptococcus pneumonia
explain the transforming principle
killed s cells + live r cells = mice died
reason : genetic material transfer from s to r
s or r strain of streptococcus pneumonia produce capsule
s cells
biochemical characterization of genetic material was given by
McCarty
Macleod
Avery
what was the experiment of McCarty Macleod & Avery
heat killed s cells (no lipid and sugar) + DNAase + r cells = no s cell characters
conclusion: transformation requires dna
who’s experiment concluded DNA was the genetic material
Hershey & Chase
Hershey & Chase conducted their experiment on
bac by bacteriophage
explain Hershey & Chase experiment
⬛grew viruses on S35 (protien) and P32 (dna) mediums seperately (radioactive)
⬛transfer to E.coli seperately
⬛viral coats seperated by blender
⬛virus seperated by centrifugation method
obs: bac was infected by radioactive P
conclusion: dna is the infecting agent