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MOLECULAR BASIS OF INHERITANCE > DNA REPLICATION > Flashcards

DNA REPLICATION Flashcards

1
Q

common method of dna replication

A

semi conservative method

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2
Q

semi conservative method takes place in ___ during ___ phase

A

nucleus, s phase

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3
Q

new deoxyribose nucleotides for replication are present in

A

nucleoplasm

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4
Q

semi conservative replication was found by

A

watson and crick

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5
Q

explain the meselson and stahl experiment

A

E.coli - 20mins and 40mins
15 NH4Cl
14 NH4Cl
centrifuge - CsCl

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6
Q

other experiment for dna replication

A

Taylor and colleagues
radioactive thymidine
Vicia faba

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7
Q

dna polymerase rate in E.coli

A

4.6*10⁶ bp in 18 sec

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8
Q

dna polymerase rate

A

2000bp per sec

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9
Q

name a dna polymerase

A

deoxyribonucleoside triphosphate

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10
Q

uses of deoxyribonucleoside triphosphate

A

⬛act as substrate
⬛ provide energy for polymerization

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11
Q

explain the mechanism of dna replication

A

ori
replication fork
leading strand 5to3
lagging strand (OKAZAKI STRANDS) 3to5, away from replication fork
dna ligase

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12
Q

okazaki fragments

A

discontinuous fragments in the lagging strand

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13
Q

failure in cell division after replication results in

A

polyploidy

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14
Q

hellicase

A

seperate the strands

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15
Q

topoisomerase

A

cut the strands

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16
Q

semi conservative method is also known as

A

semi discontinuous method

17
Q

enzyme used to cut the strands

A

topoisomerase

18
Q

enzyme used to seperate the strands

A

hellicase

19
Q

polyploidy is due to

A

failure in cell division after replication

20
Q

okazaki fragments are joined using the enzyme

A

dna ligase

MOLECULAR BASIS OF INHERITANCE (8 decks)

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