HSO and Anti-DNase B assay Table

Question 1

ASO Sensitivity

Answer 1

Lacks sensitivity - cannot detect previous/acute exposure with Group A strep

Question 2

ASO Mechanism

Answer 2

Detecting human Ab against streptomycin O toxin (looking for agglutination)

Question 3

ASO Strep Pyoderma

Answer 3

Cannot detect

Question 4

Anti-DNase B Sensitivity

Answer 4

80% more sensitive than ASO

Question 5

Anti-DNase B mechanism

Answer 5

Patient Ab blocks reagent enzyme from cleaving and prevent changing color of green solution

Question 6

Anti-DNase B Strep Pyoderma

Answer 6

Can detect

Question 7

Streptozyme assays sensitivity

Answer 7

Significant false positive and false negative in children

Question 8

Streptozyme assays mechanism

Answer 8

Detecting Ab in patient serum to agglutinate with 5 different strep exotoxins in RBC reagents