HPLC Lab Flashcards
(1 cards)
1
Q
The mobile phase is water-acetonitrile (10:90, v/v) containing 0.01 mol/L ammonium acetate.
Is this reverse or normal phase chromatography?
A
Reverse-phase chromatography typically uses a non-polar stationary phase (e.g., C18, C8 columns) and a polar mobile phase (water mixed with organic solvents like methanol or acetonitrile).