HPLC Lab Flashcards

(1 cards)

1
Q

The mobile phase is water-acetonitrile (10:90, v/v) containing 0.01 mol/L ammonium acetate.

Is this reverse or normal phase chromatography?

A

Reverse-phase chromatography typically uses a non-polar stationary phase (e.g., C18, C8 columns) and a polar mobile phase (water mixed with organic solvents like methanol or acetonitrile).

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