How We Make DNA Origami (Wagenbauer et al.)

Question 1

What are the strategies to polymerize DNA nanostructures? what are their advantages/disadvantages?

Answer 1

1) enzymatic single-strand digestion of PCR-amplified double-stranded DNA
1.5) *magnetic beads + PCR
2) asymmetric PCR
3) purifying phage-derived single-stranded genomic DNA

PCR: more customizable design but limited amount
phage DNA: less customizable, easy to produce a lot

solid-phase-synthesized DNA: for oligos/staples : commercially available

Question 2

What can you change to increase the polymerisation yield?

Answer 2

buffer conditions, for PCR, primers and polymerase.

Question 3

What is the principle that allows us to build structures with corners?

Question 4

How is it possible to build dynamical structures?

Question 5

What are the main purification techniques and their respective advantages/disadvantages?