how microscopes work

Question 1

magnification equation

Answer 1

magnification=image size/object size

Question 2

resolution

Answer 2

-how detailed the image is
-how well a microscope can differentiate between two points

Question 3

light microscope

Answer 3

-use light
-low resolution(max0.2 micrometers)
-max magnification of x1500
-used to look at whole cells or tissues

Question 4

laser scanning confocal microscopes

Answer 4

-use laser beams focused through a lens aimed at a beam splitter
-used to look at specimens tagged with a fluorescent dye
-used to look at objects at different depths
-3D images

Question 5

scanning electron microscope

Answer 5

-scan a beam of electrons across the specimen
-knocked off electrons are gathered in a cathode ray tube to form an image
-3D image
-lower resolution than TEM

Question 6

transmission electron microscope

Answer 6

-use electromagnets to focus a beam of electrons
-electron beam transmitted through the specimen
-2D image
-high resolution