HIV Case--Johns, Regel, Skildem Flashcards
HIV virus class
VI
2x (+) ssRNA
+ reverse transcriptase
Two important glycoproteins in HIV envelope
gp 41 and gp120
What are the three functional enzymes in the latent HIV particle?
reverse transcriptase
integrase
protease
What two receptors on the CD4+ cell that are bound by the HIV particle?
CXCR4
CD4
How is reverse transcription initiated?
tRNA primer tethered to reverse transcriptase
Sequences that are used to transpose viral DNA into host genome
long terminal repeats
Main trascription factor in protein synthesis of provirus
NF-kB
binds long terminal repeats, promotor region
What interacts with CD4 receptor to gain entry to T-cell?
glycoproteins
What is encoded by the gag gene?
capsid proteins
p24
Pol gene
reverse trascriptase
protease
integrase
env gene
glycoproteins
gp120 and gp41
PCR vs RT-PCR
functional uses
RT-PCR used in patients
PCR used in research
Direct vs Indirect ELISA in HIV testing usages
Direct: early in infection when antigen is high
Indirect: later in infection, shows if exposure was ever
Which test is used for HIV screening?
RT-PCR
looking for antibodies
HIV Western blot
- company HIV proteins
- patients serum washed over
- labelled antibodies added
- filmed exposed
ELISA looks for antibody against which protein?
p24
