Histotechnology Flashcards
3 essential parts of a microtome
- Block holder (chuck)
- Knife, knife carrier
- Pawl, Ratchet feedwheel, Adjustment screws
Who invented the cambridge microtome?
Paldwell Trefall (1881)
Who invented the rotary microtome?
Minot (1886)
Who invented the sliding microtome and what is the difference between the base sledge and standard sledge?
Adams
Base
Moving: Block holder
Stationary: Knife
all media, resin-embedded
Standard
Moving: knife
Starionary: Block holder
Celloidin embedded tissue
Microtome used for Electron Microscopy, indicate the knives used and the sizes
Ultrathin microtome
Glass k.= 0.5 to 1micrometer
Diamond = 500 - 1200 Armstrong
Conventional steel knife used in base sledge pr sliding microtome
Plane wedge
How many double strokes needed to do in HONING?
20 to 30 double strokes if range
30 if not range
a.Hone used in honing for best result?
b. more polishing result?
c. hone used for a much coarser than a and b
a. Belgium yellow
b. Arkansas
c. Fine corburundum
How many double strokes needed to be done in stropping? and how is it done?
40-120 strokes, Edge last toe to heel direction
Provide the angles of the ff
a. Clearance angle
b. Bevel angle
c. Perfect and optimum angle
a. 0-15
b. 27-32
c. 15
What processes can be done in Automatic Tissue Processor?
FDCI
Fixation
Dehydration
Clearing
Impregnation
Wax bath thermostat should be at least ________ the melting point
3 degrees above
In ATP (Automatic Tissue Procesor) indicate the stations and the corresponding process
ST 1-2 = Fixation (10% formalin)
ST 3-6 = Dehydration (70% to 95% of alcohol)
ST 7-8 = 2 changes of acetone (fix and dehydration)
ST 9-10 = 2 changes if chloroform and xylol (clearing)
ST 11-12 = 2 changes of paraffin (impregnation)
Flotation water bath is set at a temperature of?
45-60 degrees (~6-10 degrees lower than the melting point of the wax used)
Temperature of Oven for inflitration process and drying process
Infiltration process: 55 to 60 degrees
Drying process: 56 to 60 for 2 HOURS
