Histotechniques and Lab Safety

Question 1

What level biohazard is the laboratory?

Answer 1

Level 2

Question 2

What is one of the most important means of preventing the spread of infection?

Answer 2

hand hygiene

Question 3

How long do you flush your eyes at the eye wash station?

Answer 3

15 minutes

Question 4

What does PASS stand for referring to the fire extinguisher?

Answer 4

Pull the pin - Aim at the fire - Sweep - Side to side

Question 5

How do you calculate total magnification?

Answer 5

magnification of ocular (10x) x magnification of objective

Question 6

What connects the eyepieces with the objective lens?

Answer 6

optical tube/body tube

Question 7

What contains the housing for the fine and coarse adjustments?

Answer 7

arm

Question 8

What is the rotating wheel on the underside of the stage that allows the adjustment of the amount of light?

Answer 8

light diaphragm

Question 9

What gathers the light from the microscope and concentrates it?

Answer 9

condenser

Question 10

What is the distance between the centers of your two pupils?

Answer 10

interpupillary distance

Question 11

What are the four advantages of Koehler illumination?

Answer 11

even illumination, highest contrast and resolution, reduced specimen heating, eliminates reflection and glare

Question 12

What are the 6 histotechniques?

Answer 12

surgical removal of tissue, fixation, tissue processing, embedding, sectioning, staining

Question 13

What are the three steps in tissue processing?

Answer 13

dehydration, clearing, wax infiltration

Question 14

What chemical do they use to dehydrate the tissue?

Answer 14

ethyl alcohol

Question 15

What do they replace the alcohol with during clearing?

Answer 15

xylene

Question 16

How thin does the microtome cut the tissue?

Answer 16

4-6 um

Question 17

What stains the nuclei blue?

Answer 17

hematoxylin

Question 18

What stains the cytoplasmic content pink?

Answer 18

eosin

Question 19

What are some tissues derived from the ectoderm (outer layer)?

Answer 19

epidermis, epithelium of nasal cavities, sinuses, mouth, nervous system, posterior pituitary gland

Question 20

What are the tissues derived from the mesoderm (middle)?

Answer 20

muscle, connective tissue, epithelium of blood vessels, kidney, adrenal, etc.

Question 21

What is the microscopic study of the form and structure of NORMAL tissue?

Answer 21

histology

Question 22

What is the microscopic study of changes in diseases tissues?

Answer 22

pathology

Question 23

What is histotechnology?

Answer 23

the preparation of tissues for microscopic examination

Question 24

What are curettings?

Answer 24

scrapings

Question 25

How do they accession specimens for identification?

Answer 25

lab pathology number

Question 26

What are the pieces of dissected tissue placed in?

Answer 26

small plastic cassettes

Question 27

how is the kidney cut?

Answer 27

BIsected

Question 28

how is the spleen cut?

Answer 28

several times

Question 29

How long are species preserved in formalin?

Answer 29

approximately 3 months

Question 30

What is the preservative used for fixation?

Answer 30

10% neutral buffer formalin

Question 31

What is the decomposition of tissue by bacteria, fungi, or viruses?

Answer 31

putrefaction

Question 32

What are the two methods of fixation?

Answer 32

physical (freezing), chemical (fluid fixative)

Question 33

What is the aim of tissue processing?

Answer 33

to make fixed tissues ready for cutting into thin sections

Question 34

How long does typical tissue processing take?

Answer 34

over 15 hours (4:30pm - 7am)

Question 35

What is the job of paraffin wax?

Answer 35

to fill all the spaces within the tissue so that cellular components are adequately supported

Question 36

Which stain is basophilic?

Answer 36

blue - hematoxylin

Question 37

What are the three stages of staining tissues?

Answer 37

dewaxing, staining, permanent preparation (mount)