HISTOPATHOLOGY

Question 1

Fats, Mucin, Glycogen (FMG)

Answer 1

Formaldehyde

Question 2

Most widely used for Electron Microscopy
Best fixative for Enzyme Histochemistry

Answer 2

Glutaraldehyde

Question 3

Glutaraldehyde concentration for small tissues

Answer 3

2.5%

Question 4

Glutaraldehyde concentration for large tissues

Answer 4

4.0%

Question 5

4% concentration; acid mucopolysaccharides

Answer 5

Lead fixative

Question 6

Best for mucopolysaccharides; Nuclear proteins
Best fixation: 3 degC for 12-18 hours

Answer 6

Newcomer’s Fluid

Question 7

50-100x volume of fat elements

Answer 7

Musem

Question 8

Recommended for trichome staining;
Made up of Mercuric Chloride stock solution with Glacial Acetic Acid before use;
Connective tissue, Liver, Spleen, Nuclei

Answer 8

Zenker’s Fluid

Question 9

Most rapid fixative;
Chromosomes; Lymph Glands; Urgent Biopsies; NISSL granules

Answer 9

Carnoy’s Fluid

Question 10

Most common fixative;
5-7% saturated aqueous solution
Tissue photography
Renal Tissue; Connective tissue; Muscle; Fibrin

Answer 10

Mercuric Chloride

Question 11

Micro-anatomical & Cytological Fixative
Pituitary gland; Bone marrow; Blood-containing organs (Spleen & Liver)

Answer 11

Helly’s Fluid/ Zenker’s Formol

Question 12

MMCCRG:
Mitochondria
Mitotic Figures
Chromatin
Colloid containing tissues
RBCs
Golgi Bodies

Hemosiderin (iron pigment containing hemoglobin)

Answer 12

Regaud’s Fluid

Question 13

Consist of picric acid;
Preserve glycogen;
Not for kidney tissues;
Embryo

Answer 13

Bouin’s Fluid

Question 14

Less slower than Regaud’s Fluid
Early degenerative process and tissue necrosis (pathological cell death)
Demonstrate Rickettsiae spp

Answer 14

Orth’s Fluid

Question 15

Microanatomical & Cytological Fixative
For tumor biopsies of the skin

Answer 15

Heidenhain’s Susa Fluid

Question 16

Fixes elastic fibers & iron pigments
Post-mortem changes;
Research tissues;
Surgical storage tissues

Answer 16

10% Neutral Buffered Formalin

Question 17

General post-mortem changes;
CNS tissues

Answer 17

10% Formol Saline

Question 18

Routine post-mortem changes
Lipids

Answer 18

Formol Corrosive/ Formol Sublimate

Question 19

Glycogen;
Better & less messy than Bouin’s Fluid

Answer 19

Brasil’s Alcohol Picroformol Solution

Question 20

Nuclear structures

Answer 20

Flemming’s Fluid with Glacial Acetic Acid

Question 21

Cytoplasmic structures
Consist of chromic and osmic acid

Answer 21

Flemming’s fluid without glacial acetic acid