HISTOPATHOLOGY Flashcards
Fats, Mucin, Glycogen (FMG)
Formaldehyde
Most widely used for Electron Microscopy
Best fixative for Enzyme Histochemistry
Glutaraldehyde
Glutaraldehyde concentration for small tissues
2.5%
Glutaraldehyde concentration for large tissues
4.0%
4% concentration; acid mucopolysaccharides
Lead fixative
Best for mucopolysaccharides; Nuclear proteins
Best fixation: 3 degC for 12-18 hours
Newcomer’s Fluid
50-100x volume of fat elements
Musem
Recommended for trichome staining;
Made up of Mercuric Chloride stock solution with Glacial Acetic Acid before use;
Connective tissue, Liver, Spleen, Nuclei
Zenker’s Fluid
Most rapid fixative;
Chromosomes; Lymph Glands; Urgent Biopsies; NISSL granules
Carnoy’s Fluid
Most common fixative;
5-7% saturated aqueous solution
Tissue photography
Renal Tissue; Connective tissue; Muscle; Fibrin
Mercuric Chloride
Micro-anatomical & Cytological Fixative
Pituitary gland; Bone marrow; Blood-containing organs (Spleen & Liver)
Helly’s Fluid/ Zenker’s Formol
MMCCRG:
Mitochondria
Mitotic Figures
Chromatin
Colloid containing tissues
RBCs
Golgi Bodies
Hemosiderin (iron pigment containing hemoglobin)
Regaud’s Fluid
Consist of picric acid;
Preserve glycogen;
Not for kidney tissues;
Embryo
Bouin’s Fluid
Less slower than Regaud’s Fluid
Early degenerative process and tissue necrosis (pathological cell death)
Demonstrate Rickettsiae spp
Orth’s Fluid
Microanatomical & Cytological Fixative
For tumor biopsies of the skin
Heidenhain’s Susa Fluid
Fixes elastic fibers & iron pigments
Post-mortem changes;
Research tissues;
Surgical storage tissues
10% Neutral Buffered Formalin
General post-mortem changes;
CNS tissues
10% Formol Saline
Routine post-mortem changes
Lipids
Formol Corrosive/ Formol Sublimate
Glycogen;
Better & less messy than Bouin’s Fluid
Brasil’s Alcohol Picroformol Solution
Nuclear structures
Flemming’s Fluid with Glacial Acetic Acid
Cytoplasmic structures
Consist of chromic and osmic acid
Flemming’s fluid without glacial acetic acid
