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Histopath > Histopath Shifting exam > Flashcards

Histopath Shifting exam Flashcards

1
Q

removing a bit of tissue from living being

A

biopsy

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2
Q

removing a large tissue or organ from dead body

A

autopsy

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3
Q

destruction of tissue due to its own enzyme

A

autolysis

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4
Q

destruction of tissue due to environmental bacteria and microorganism

A

putrefaction

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5
Q

tissues are collected and fixed immediately in a ______.

A

10% neutral buffered formalin

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6
Q

____ the volume of fixative in ratio to the size of the specimen.

A

3 times | 1:3 (specimen:volume of fixative)

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7
Q

cutting the specimen into 3 or more parts

A

loafing

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8
Q

sample is transported at ______.

a. body temp
b. room temp
c. ref temp

A

b. room temperature

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9
Q

must not be placed into any other solution or dry container except for _____.

A

frozen section

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10
Q

crucial parts of pathologic examination which is done by a pathologist. (2)

A

dissection and selection of sections

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11
Q

Cartilaginous, bony, and hard tissues are placed in the ______ solution for ____ days.

A

decalcifying solution, 1-7 days

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12
Q

_____ of samples is integrated into the grossing system to evaluate the margin of resection in microscopy.

A

inking

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13
Q

the thickness of cutting the specimen is no more than ___.

A

5mm

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14
Q

Process: Removal of water from aqueous-fixed tissue.

A

dehydration

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15
Q

A reagent that can mix both alcohol and wax. Bridge for alcohol and wax.

A

Organic solvent xylene/ Xylene

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16
Q

Process: Replacing the dehydrant with a substance that will be miscible with the embedding medium.

A

clearing

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17
Q

Process: Final xylene is replaced with molten wax which infiltrates the tissue.

A

Impregnation or infiltration

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18
Q

tissue processing is routinely done in an instrument called ______.

A

Automated Tissue Processor

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19
Q

a carousel type semi-enclosed tissue processor with 12 stations.

A

Leica TP 1020 Tissue Processor

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20
Q

oven for ____ at ____ C.

A

1 hour, 70 C

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21
Q

Set on to the _____ neatly and in order for _______ duration of time.

A

cold plate, 30 minutes

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22
Q

An instrument used to cut section from tissue embedded in paraffin wax.

A

microtome

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23
Q

Use the ______ to secure tissue block.

A

lever

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24
Q

Used to move the block downward across the blade.

A

hand wheel

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25
Q

In order for the wheel to be turned, it must be turned downward and the handle pulled outward.

A

safety knob

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26
Q

Blade can be adjusted or removed using this knob.

A

Blade adjustment knob

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27
Q

when it needs to be cleaned, use only a brush on an ______ motion.

A

upward

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28
Q

it is used to move forward and backward.

A

Front knob

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29
Q

It is used for lateral adjustments.

A

mabhab

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30
Q

It changes the angle of the knife holder.

A

Knife angle knob

31
Q

Removing the outer layer of wax in the block.

A

Trimming

32
Q

Place the ribbon on warm water bath at ___ C.

A

45 C

33
Q

Removing of excess paraffin wax.

A

Deparaffinization

34
Q

A nuclear stain which stains cell nucleus.

A

Hematoxylin stain

35
Q

Removes excess stain and define nuclei.

A

acid alcohol

36
Q

It serves as bluing agent.

A

Ammonia

37
Q

It stains the extracellular matrix and cytoplasm in pink.

A

Eosin

38
Q

Pulls excess eosin from the tissue.

A

water

39
Q

Provides permanent preservation of H&E stained tissue sections; Protection from dust.

A

Protective cover slip

40
Q

It helps the specimen to be sliced more easily.

a. warm water bath
b. ice bath

A

b. ice bath

41
Q

Helps smooth out any wrinkles from slicing the specimen.

a. warm water bath
b. ice bath

A

a. warm water bath

42
Q

Used to stain paps smear.

A

paps stain

43
Q

Most important stain in the practice of cytopathology.

A

paps stain

44
Q

How many dyes are there in pap stain?

A

5 dyes

45
Q

What are the 2 cytoplasmic counterstains in pap stain?

A

OG-6/Orange G-6 and EA-50/Eosin Azure-50

46
Q

What stain that stains cell nuclei in blue?

A

hematoxylin

47
Q

What is the commonly used regressive technique?

A

Harris hematoxylin

48
Q

First acidic counter stain.

a. Orange G-6
b. Eosin Azure-50

A

Orange G-6

49
Q

Monochrome stain.

a. Orange G-6
b. Eosin Azure-50

A

Orange G-6

50
Q

Stains protein keratin in orange.

a. Orange G-6
b. Eosin Azure-50

A

Orange G-6

51
Q

Second counter stain.

a. Orange G-6
b. Eosin Azure-50

A

Eosin Azure-50

52
Q

Commonly used cytoplasmic stain.

a. Orange G-6
b. Eosin Azure-50

A

Eosin Azure-50

53
Q

Polychromatic stain (mixture of ___ number stains).

a. Orange G-6
b. Eosin Azure-50

A

Eosin Azure-50, 3 stains

54
Q

Stains the cytoplasmic of cells in the shades of pink or green depending on the pH.

A

Eosin Y

55
Q

Helps prepare the sample for the uptake of nuclear dye. (paps)

A

fixation

56
Q

Prepares the cell sample for uptake of the counter stain. (paps)

A

dehydration

57
Q

An agent that is a Refractive index similar to glass slides, cover slip, and mounting medium.

A

Xylene

58
Q

Injury caused by freezing of the skin and underlying tissues.

A

frostbite

59
Q

Advanced formulation that is designed to support tissue during cryotomy.

A

Tissue freezing medium

60
Q

Can set to very high or very low depending on the specimen to be cut out. (cryo)

A

Thickness indicator

61
Q

To extra freeze the samples.

A

PE button

62
Q

What is the temperature range in cryostat?

A

-20C to -30C

63
Q

To image clearly the samples. (cryo)

A

Light button

64
Q

Magnetic stainless steel disc for mounting. (cryo)

A

Specimen disc

65
Q

To secure the tissue block. (cryo)

A

Mounting area

66
Q

Where does shaving off the block? (cryo)

A

stage

67
Q

To flatten the sliced tissue. (cryo)

A

anti roll cover

68
Q

To lock and unlock the blade. (cryo)

A

blade level

69
Q

To manipulate the stage sideways. (cryo)

A

stage lock

70
Q

Moves the mounting area microscopically Away or towards the blade. (cryo)

A

one arrow button

71
Q

Moves the mounting area at a greater distance. (cryo)

A

two arrow button

72
Q

Moves the mounting area on upward and downward movements. (cryo)

A

hand wheel

73
Q

Use this bar to immediately freeze the tissue sample.

A

Heat extraction bar

74
Q

Use this technique if the sample is starting to roll. (cryo)

A

brush technique

Histopath (3 decks)

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