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Histopath Shifting exam Flashcards

1
Q

removing a bit of tissue from living being

A

biopsy

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2
Q

removing a large tissue or organ from dead body

A

autopsy

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3
Q

destruction of tissue due to its own enzyme

A

autolysis

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4
Q

destruction of tissue due to environmental bacteria and microorganism

A

putrefaction

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5
Q

tissues are collected and fixed immediately in a ______.

A

10% neutral buffered formalin

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6
Q

____ the volume of fixative in ratio to the size of the specimen.

A

3 times | 1:3 (specimen:volume of fixative)

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7
Q

cutting the specimen into 3 or more parts

A

loafing

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8
Q

sample is transported at ______.

a. body temp
b. room temp
c. ref temp

A

b. room temperature

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9
Q

must not be placed into any other solution or dry container except for _____.

A

frozen section

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10
Q

crucial parts of pathologic examination which is done by a pathologist. (2)

A

dissection and selection of sections

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11
Q

Cartilaginous, bony, and hard tissues are placed in the ______ solution for ____ days.

A

decalcifying solution, 1-7 days

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12
Q

_____ of samples is integrated into the grossing system to evaluate the margin of resection in microscopy.

A

inking

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13
Q

the thickness of cutting the specimen is no more than ___.

A

5mm

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14
Q

Process: Removal of water from aqueous-fixed tissue.

A

dehydration

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15
Q

A reagent that can mix both alcohol and wax. Bridge for alcohol and wax.

A

Organic solvent xylene/ Xylene

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16
Q

Process: Replacing the dehydrant with a substance that will be miscible with the embedding medium.

A

clearing

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17
Q

Process: Final xylene is replaced with molten wax which infiltrates the tissue.

A

Impregnation or infiltration

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18
Q

tissue processing is routinely done in an instrument called ______.

A

Automated Tissue Processor

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19
Q

a carousel type semi-enclosed tissue processor with 12 stations.

A

Leica TP 1020 Tissue Processor

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20
Q

oven for ____ at ____ C.

A

1 hour, 70 C

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21
Q

Set on to the _____ neatly and in order for _______ duration of time.

A

cold plate, 30 minutes

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22
Q

An instrument used to cut section from tissue embedded in paraffin wax.

A

microtome

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23
Q

Use the ______ to secure tissue block.

A

lever

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24
Q

Used to move the block downward across the blade.

A

hand wheel

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25
In order for the wheel to be turned, it must be turned downward and the handle pulled outward.
safety knob
26
Blade can be adjusted or removed using this knob.
Blade adjustment knob
27
when it needs to be cleaned, use only a brush on an ______ motion.
upward
28
it is used to move forward and backward.
Front knob
29
It is used for lateral adjustments.
mabhab
30
It changes the angle of the knife holder.
Knife angle knob
31
Removing the outer layer of wax in the block.
Trimming
32
Place the ribbon on warm water bath at ___ C.
45 C
33
Removing of excess paraffin wax.
Deparaffinization
34
A nuclear stain which stains cell nucleus.
Hematoxylin stain
35
Removes excess stain and define nuclei.
acid alcohol
36
It serves as bluing agent.
Ammonia
37
It stains the extracellular matrix and cytoplasm in pink.
Eosin
38
Pulls excess eosin from the tissue.
water
39
Provides permanent preservation of H&E stained tissue sections; Protection from dust.
Protective cover slip
40
It helps the specimen to be sliced more easily. a. warm water bath b. ice bath
b. ice bath
41
Helps smooth out any wrinkles from slicing the specimen. a. warm water bath b. ice bath
a. warm water bath
42
Used to stain paps smear.
paps stain
43
Most important stain in the practice of cytopathology.
paps stain
44
How many dyes are there in pap stain?
5 dyes
45
What are the 2 cytoplasmic counterstains in pap stain?
OG-6/Orange G-6 and EA-50/Eosin Azure-50
46
What stain that stains cell nuclei in blue?
hematoxylin
47
What is the commonly used regressive technique?
Harris hematoxylin
48
First acidic counter stain. a. Orange G-6 b. Eosin Azure-50
Orange G-6
49
Monochrome stain. a. Orange G-6 b. Eosin Azure-50
Orange G-6
50
Stains protein keratin in orange. a. Orange G-6 b. Eosin Azure-50
Orange G-6
51
Second counter stain. a. Orange G-6 b. Eosin Azure-50
Eosin Azure-50
52
Commonly used cytoplasmic stain. a. Orange G-6 b. Eosin Azure-50
Eosin Azure-50
53
Polychromatic stain (mixture of ___ *number* stains). a. Orange G-6 b. Eosin Azure-50
Eosin Azure-50, 3 stains
54
Stains the cytoplasmic of cells in the shades of pink or green depending on the pH.
Eosin Y
55
Helps prepare the sample for the uptake of nuclear dye. (paps)
fixation
56
Prepares the cell sample for uptake of the counter stain. (paps)
dehydration
57
An agent that is a Refractive index similar to glass slides, cover slip, and mounting medium.
Xylene
58
Injury caused by freezing of the skin and underlying tissues.
frostbite
59
Advanced formulation that is designed to support tissue during cryotomy.
Tissue freezing medium
60
Can set to very high or very low depending on the specimen to be cut out. (cryo)
Thickness indicator
61
To extra freeze the samples.
PE button
62
What is the temperature range in cryostat?
-20C to -30C
63
To image clearly the samples. (cryo)
Light button
64
Magnetic stainless steel disc for mounting. (cryo)
Specimen disc
65
To secure the tissue block. (cryo)
Mounting area
66
Where does shaving off the block? (cryo)
stage
67
To flatten the sliced tissue. (cryo)
anti roll cover
68
To lock and unlock the blade. (cryo)
blade level
69
To manipulate the stage sideways. (cryo)
stage lock
70
Moves the mounting area microscopically Away or towards the blade. (cryo)
one arrow button
71
Moves the mounting area at a greater distance. (cryo)
two arrow button
72
Moves the mounting area on upward and downward movements. (cryo)
hand wheel
73
Use this bar to immediately freeze the tissue sample.
Heat extraction bar
74
Use this technique if the sample is starting to roll. (cryo)
brush technique

Histopath (3 decks)

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