Histopath Formative Assessments Flashcards
Formalin diffuses into the tissue at the rate of ___ mm per hour.
a 1
b 3
c 2
d 1.5
a. 1
Brain is usually suspended whole in 10% buffered formalin for 2 – 3 days.
True
False
false
Larger tissues require more fixatives and longer fixation time.
True
False
true
To maintain an adequate fixation time of 4 to 6 hours, the recommended size of the tissue no more than 4 mm. thick.
True
False
true
All of the following are commonly used dehydrating agents. EXCEPT:
Xylene
Ethyl alcohol
Acetone
Triethyl phosphate
xylene (?)
Recommended for surface decalcification of the tissue blocks.
Acid alcohol
Mercuric acid
HCl
Picric acid
HCl
Solid tissues should NEVER be allowed to air dry.
True
False
true
Recommended for routine dehydration of tissues is methanol.
True
False
false
The most commonly used clearing agent is Xylene.
True
False
true
Viscosity also affects the speed of penetration of the clearing agent.
True
False
true
Most carcinogenic clearing agent and may damage the bone marrow.
Chloroform
Xylene
Carbon tetrachloride
Benzene
benzene
Impregnation is AKA Blocking.
True
False
false
Dehydration should NOT dehydrate even fatty tissue.
True
False
false
The clearing agent will make microscopic tissue preparations TRANSPARENT due to their VERY LOW index of refraction.
True
False
false
Embedding AKA Casting.
True
False
true
