histopath mounting and staining

Question 1

tissue components made visible by microscopic sections

Answer 1

Stainingstaining

Question 2

purified form of coloring agnet or crude dye generally applied in aqueaous solution

Answer 2

histologic stain

Question 3

acidic and basic part of cell

Answer 3

nucleus acidic, cutoplasm basic

Question 4

chemical compound that reacts with stain to form insoluble colored precipitate on the tisse and make up the staining reaction possible

Answer 4

mordant

Question 5

poorly permeale o most staining solution

Answer 5

paraffin eax

Question 6

methods of staining

Answer 6

1. direct
2. indirect
   3.progressive
3. regressive
4. differentiation
5. metachromatic
   7.metallic
6. vital
7. intravital
8. supravivtal

Question 7

only one dye used and washed away every 30-60 seconds prior to drying and exdamination

Answer 7

direct staining

Question 8

action of dye is intensified by another agent or mordant

Answer 8

indirect staining

Question 9

mordant combines with dye to form a lake to form a

Answer 9

tissue-mordant-dye-complex

that is insoluble in aqeous and alcoholic solvent

Question 10

example of mordant

Answer 10

potassium alum with hematoxylin in ehrlich;s hematoxylin and iron ni weigert’s hematoxylin

Question 11

accelerates reaction of chemical union of tissue and dye

Answer 11

accentuator

Question 12

example of accentuator

Answer 12

mthyklene blue and phenol in carbol thionine and fuschin

Question 13

tissue elements are staining in definite sequrnce

Answer 13

progressive staining

Question 14

tissue is overstainied to obliterate cellular details tas dedecolorize excess stain from unwanted parts till desired intensity of color is obrained

Answer 14

regressive staining

Question 15

example oof regressive staining

Answer 15

hematoxylin and eosin staining

Question 16

used for micranatomical studies of tissues, overstianing the nuclei then removing te excess color by acid differentiation

Answer 16

regressive staning

Question 17

selective removal of ecess stain

Answer 17

differential stain

Question 18

differentiator for both acidic and basic dye by simply dissolving dye

Answer 18

alcohohol

Question 19

detects abnormalities in proportion of different wbc in bloof

Answer 19

alcohol

Question 20

oxidize hematoxylin to colorless compount para madeoclorize tissue

Answer 20

iron alum

Question 21

stainins tissue in color shades similar to color of dye itself

Answer 21

metachromatic staining

Question 22

staining cartilage, ct, epithelial mucus, mast cell granules and amyloid

Answer 22

metachromatic stianingq

Question 23

example of metachromatic staining

Answer 23

azure/toluidine blue

Question 24

specific tissue elements are shown via colorless solution of metallic salts which are then reduced by tisses, producing an opaque usually black deposit

Answer 24

metallic impregnation

Question 25

selective staining of living cell constituents demonstrating cytoplasmic structure by phagocytosis of dye particle or preexisting cellular components

Answer 25

vital staining

Question 26

staining of mitochondria

Answer 26

janus green

Question 27

staing of reticuendothelial

Answer 27

trypan blue or propidium iodine

Question 28

injection of dye into any part of animal body

Answer 28

intravital stainingavi

Question 29

intraviotal staining example

Answer 29

lithium, carmine, india ink

Question 30

examine living cells that have been removed from organism

Answer 30

supravital

Question 31

supravital stains example

Answer 31

newmethylene blue, brilliant cresyl blue

Question 32

dilution ratio of supravital stainig

Answer 32

1;5,000 to 1;50,000

Question 33

best vital dye

Answer 33

neutral red

Question 34

cornerstone of tissue based diagnosis

Answer 34

hematoxylin and eosin staining

Question 35

confirms basic tissue type and helps localize lesion

Answer 35

hematoxyin and eosin staining

Question 36

inhibits hematoxylin

Answer 36

osmic acid solution