Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

MTAP > HISTOPATH > Flashcards

HISTOPATH Flashcards

1
Q

Specimen Retention

A

1 month to 1 year

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Retention of Tissue Block

A

3 years to 10 years

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Retention of:
Slides
Records
Result Forms

A

Indefinite
Permanent
Triplicate

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

FISCAM

A

Forensic Pathology
Immunohistopathology
Surgical Pathology
Cytology
Autopsy
Molecular Biopsy

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Thickness:
Whole mount
Sectioning

A

0.2 - 0.5mm
3.0 - 5.0mm

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Microscopic exam of cells from different body sites

A

Diagnostic Cytology

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Microscopic exam of cells that have been desquamated from epithelial surfaces

A

Exfoliative cytology

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Histotechniques?

A

FDCIETSSML
Fixation, (Decalcification), Dehydration, Clearing, Impregnation, Embedding, Trimming, Section-Cutting, Staining, Mounting, Labelling

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Also referred to as “Dissection”/”Separation”; Disadvantage: anatomical relationship is destroyed

A

TEASING/DISSOCIATION

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

FACTORS AFFECTING FIXATION/PRESERVATION

A
  1. Size and nature/type of tissue
  2. Volume of tissue: 1:20x volume of tissue
  3. pH: 6-8
  4. Temperature: Room Temperature (20-22OC)
  5. Osmolality: slightly hypertonic solution
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

fixative for fats, mucin, glycogen

A

FORMALDEHYDE

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

most widely used for Electron Microscopy; Best fixative for enzyme histochemistry

A

GLUTARALDEHYDE

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

fixative for acid mucopolysaccharide; connective tissue

A

LEAD FIXATIVE

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

fixative for mucopolysaccharide; nuclear proteins

A

NEWCOMER’S FLUID

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

fixative for connective tissue, liver, spleen, nuclei; Trichome Staining; Made up of mercuric chloride stock solution in which glacial acetic is added before use

A

ZENKER’S FLUID

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

MOST RAPID FIXATIVE

A

CARNOY’S FLUID

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

MOST COMMON FIXATIVE

A

MERCURIC CHLORIDE

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

Fixative for Hemosiderin; Better and Rapid compared to Orth’s; “Moller’s Fluid”

A

Regaud’s Fluid

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

early degenerative processes and tissue necrosis; demonstrate Rickettsiae spp.

A

ORTH’S FLUID

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

fixative for tumor biopsies of the skin

A

HEIDENHAIN’S SUSA FLUID

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

routine post mortem changes; fixes elastic fibers and iron pigments

A

10% Neutral Buffered Formalin

22
Q

Fixes cytoplasmic structure; consists of Osmic and Chromic acid

A

FLEMMING’S FLUID WITHOUT GLACIAL ACETIC ACID

23
Q

solidifies at 17 deg C; precipitates chromosomes and chromatin

A

Glacial Acetic Acid

24
Q

fixative and dehydrant; brain damage; rabies diagnosis; suitble for urgent biopsies

A

ACETONE

25
Q

fixative and dehydrant; ideal for small bone fragments

A

ALCOHOL

26
Q

recommended fixative for carbohydrates; acts as decalcified for minute bone spicules

A

CHROMIC ACID

27
Q

Coagulates mucus, fixative for sputum

A

Gendre’s fluid

28
Q

fixative for ultrathin type microtom; pale yellow powder dissolved in water (6% concentration; kept at 20 degC)

A

OSMIC ACID/OSMIUM TETROXIDE

29
Q

FACTORS AFFECTING DECALCIFICATION/DEMINERALIZATION

A
  1. Hard, delicate, tough specimens
  2. Volume of tissue (1:20x volume of the tissue)
  3. Time of Decalcification: 24-48 hours
  4. pH: Acidic
  5. Temperature: Room Temperature (18-30OC)
  6. Concentration
30
Q

Most rapid decalcifier

A

Phloroglucin Nitric Acid

31
Q

General Post-Mortem Changes

A

10% FORMOL SALINE

32
Q

Most common decalcifier; Rapid, routine uses; Urgent Biopsies; De Castro’s Fluid

A

Nitric Acid

33
Q

From Formaldehyde; Routine Post-mortem research

A

Formic Acid

34
Q

decalcifier for surface tissue block; slower action; greater distortion of tissues

A

Hydrochloric acid

35
Q

Permits good nuclear staining; very weak decalcifier

A

Trichloroacetic acid

36
Q

Composition of Perenyi’s fluid

A

0.5% Chromic Acid
1.0% Nitric Acid
Absolute Alcohol

37
Q

Dehydrant: Most common, cheap and not poisonous

A

Ethanol

38
Q

Dehydrant for Blood and Tissue Films and Smears

A

Methanol

39
Q

Substitute for Ethanol; miscible with water, expensive, and does not harden tissue

A

Isopropanol

40
Q

fixative and dehydrant; volatile; suitable for urgent biopsies; hardens tissue than ethanol

A

Acetone

41
Q

dehydrant that consists of PEG

A

Cellosolve

42
Q

Dehydrant and Clearing agent but expensive and toxic

A

Dioxane/Diethylene Dioxide

43
Q

Dehydrant and clearing agent; causes conjunctival irritation

A

Tetrahydrofuran

44
Q

Most common clearing agent

A

Xylene

45
Q

Clearing time for less than 3mm? greater than 5mm?

A

15-30 mins
30-40 mins

46
Q

clearing agent that is suitable for urgent biopsies; associated with aplastic anemia

A

Benzene

47
Q

Substitute of Xylene and Benzene

A

Toluene

48
Q

Clearing agent that can be used for paraffin and celloidin embedded tissue

A

Cedarwood Oil

49
Q

Clearing for Lymph Nodes, Embryo, Nervous tissue; toxic to livers

A

Chloroform

50
Q

Substitute of cedarwood oil

A

Terpineol

51
Q

Tissue softener for fingernails and uterine curettings

A

Lendrum’s fluid

52
Q

Tissue softener: tissue is soapy and swollen

A

Molliflex

MTAP (2 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions