Histology (Staining/Microscopes)

Question 1

True or False

Hematoxylin provides a purple pigment for cytoplasm. Eosin provides a pink pigment for the nucleus.

Answer 1

Hematoxylin provides a purple pigment for NUCLEUS. Eosin provides a pink pigment for the CYTOPLASM.

Question 2

Which of the following is not a basic dye?

Methyl green, Methylene blue, Pyronine G and Aniline blue

Answer 2

Aniline Blue

Question 3

Which of the following is not a acid dye?

Orange G, Eosin, Methylene blue, Aniline blue or Acid Fuchsin

Answer 3

Methylene Blue

Question 4

What is the reason Hematoxylin can “act” like a base?

Answer 4

Mordant binds to the tissue

Question 5

What is an example of metachromasia?

Answer 5

Toluidine blue staining cartilage ground substance or mass cell granules

Question 6

Histochemistry is used to study the presence of specific tissue constituents or antigens by using monoclonal antibodies.

True or False

Answer 6

False, this is immunocytochemistry

Question 7

What is histochemistry?

Answer 7

Study of chemistry of cells and tissues

Example: Check for iron in cell levels or lipids

Question 8

Sudan dyes are used for what type of tissues?

Answer 8

Adipose, since standard methods with alcohol harshly clears fat cells.

Question 9

What color does Schiff Reagent form?

Answer 9

Pinkish color after reacting aldehyde groups

Question 10

Real Image

Answer 10

when object is placed outside focal point. Creates an inverted image that is projectable and differs in size from object.

Question 11

Virtual Image

Answer 11

when object is placed within focal point. Not inverted. Not projectable. Magnifiable.