Histology Lecture 2: Microscopy

Question 1

Answer 1

Refractive Index

Question 2

Refractive Index of Air, by definition

Answer 2

1

Question 3

a measure of how much a lens bends light waves, measured in diopters

Answer 3

Refractive Power

Question 4

Diopter1 meter divided by the focal length of the lens

Answer 4

1 diopter

Question 5

The point through which all parallel rays of light will pass
after passing through each part of the lens.

Answer 5

Focal Point

Question 6

The distance from the center of the lens to the focal point.

Answer 6

Focal Length

Question 7

Formed when the object is placed outside the focal point

Answer 7

real image

Question 8

Inverted (always), can be projected, and can be magnified

Answer 8

real image

Question 9

formed when the object is placed inside the focal point. Not inverted, cannot be projected, can be magnified

Answer 9

virtual image

Question 10

the ability of a microscope to distinguish two points as separate

Answer 10

resolution

Question 11

Answer 11

Equation for Resolving Power

Question 12

can be used to examine unstained (and live) cells and tissues by converting phase shifts (invisible to the human eye) in light passing through a specimen to brightness changes (visible to the human eye) in the image

Answer 12

Phase Contrast Microscope

Question 13

Increases optical resolution and contrast by means of adding a pinhole placed at the confocal plane of the lens to eliminate out-of-focus light. Enables reconstructing of 3D images with computers.

Answer 13

Confocal Scanning Microscope