Histology and Embryology

Question 1

Label parts A, B, C, and D of the microscope:

Answer 1

Question 2

What type of microscope was used?

Answer 2

Phase contrast microscopy:

allows observation of living non-stained structures

Question 3

What type of microscope was used?

Answer 3

Fluorescence microscopy

Question 4

Compare the type of microscopes used:

Answer 4

TEM on the left; SEM on the right

Question 5

What are the three specific conditions must specimens meet in order to be observed under a brightfield microscope &/or a TEM?

Answer 5

1. Be well perserved
2. Be sufficiently thin
3. Have enough contrast to observe details

Question 6

What is the process for observing histological specimens?

Answer 6

1. Fixation - 10% buddered formalin
2. Dehydration - ascending % of alcohol
3. Clearing - xylene
4. Infiltration
5. Embedding - paraffin

Question 7

A. Acid dyes stain______ (basic/acidic) structures ________ (blue/red)

B. What is considered an acidic dye?

Answer 7

A. Basic; blue (Collagen, protein amines)

B. Eosin

Question 8

A. Basic dyes stain______ (basic/acidic) structures ________ (blue/red)

B. What is considered an basic dye?

Answer 8

A. Acidic; red (DNA, RNA, acid proteins)

B. Hematoxylin

Question 9

Label the following cell:

Answer 9

Pancratic acinar cell

Question 10

______ stain is used for the basement membrane and to localize carbohydrates

Answer 10

PAS (periodic acid Schiff)

Question 11

Compare the two types of staining:

Answer 11

H & E stain on the left

PAS stain on the right

of goblet cells