Histology Flashcards
Resolution
the smallest distance at which two points can be distinguished from each other.
Resolution of the - Human eye - Bright-Field Microscope - TEM
-Human eye: 0.2 mm • Bright-field microscope: 0.2 µm • TEM: 2-3 nm
TEM allows to examine internal cellular structure
Electron-dense Electron-lucent
what region is shown by the colored dots?

TEM terms: Electron-dense (red), Electron-lucent (purple)
What is indicated by the blue arrow

BASIC stains, e.g. hematoxylin (blue), interact with acidic cellular components (basophilic, e.g. nucleic acids)
What is indicated by the red arrow

ACIDIC stains, e.g. eosin (pink), interact with basic cellular components (acidophilic, e.g. cytoplasm)
Name the steps in the preparation of tissue
- Fixation chemical physical
- Dehydration water→ethanol →xylene
- Embedding paraffin
- Sectioning
This picture is an example of what type of staining

Acidic components:
- Stain with basic dyes (e.g. hematoxylin)
- They are “basophilic” (typically blue)
- Examples: Nucleic acids (DNA & RNA) Dye+Nucleic acid
This picture is an example of what type of staining

Basic components:
- Stain with acidic dyes (e.g. eosin)
- They are “acidophilic” (typically pink/red)
- Examples: Cytoplasm Collagen fibers Examples of cell/tissue staining Collagen+Dye
What is this and what cell components does it stain

Hematoxylin
Stains basophilic cell components
Negatively charged anionic tissue - RNA+DNA (Acidic)
What is this and what cell components does it stain

Eosin
Stains acidophilic cell components - positively charged
Basic strcutures - like cytoplasm and collagen fibers
What stains are included in this pic

Hematoxylin and Eosin
What is this stain and what is it used for

Orcein – elastic fibers
What is this stain and what is it used for

Sudan black, osmium tetroxide - lipids
What is this stain and what is it used for

Silver stainreticular fibers
Name this stain and what its used for

PAS (Periodic Acid – Schiff) – carbohydrates
Name this stain and what its used for

Immunocytochemistry – specific Antibody-based interactions
Name the stain and what its used for

Trichrome stains – collagen fibers (blue or green)
Freeze fracture & freeze etch is used for what

SEM & TEM
What does this picture represent and what is its stain

Mucus, PAS stain
What does this picture represent and what is its stain

Lipids
Sudan black
What is represented in this picture

Red blood cell (RBC or erythrocyte) can be used as a “biological yardstick”. RBC’s diameter is 7-8 µm.
What is represented in this picture

Artificial spaces between the cells (tissue shrinkage).
What is represented in this picture

“Empty cells” due to the loss of substances.
What is represented in this picture

Wrinkles in the sectioned tissue
What type of tissue is this and in what part of the body is it located

Epithelial cells in the kidney
What type of tissue is this and in what part of the body is it located

Dorsal root ganglion cells
What type of tissue is this and in what part of the body is it located

Smooth muscle cells of the small intestine
What structures are represented in this picture

Electron micrograph of a plasma membrane (plasmalemma) that surrounds the cell
It contains phospholipids, cholesterol and proteins
What process is represented in the picture

Ingestion of large particles results in the formation of an intracellular vacuole (phagosome)
What process is occurring in picture

Pinocytosis
Label the letters depicted in the image

Ps: phagosomes
B: Bacteria
rER: rough endoplasmic reticulum
M: Mitochondria
NE: Nuclear envelope
L: Lysosome
Label the letters depicted in the image and identify what process is occurring

SG: Secretory granules
rER: rough endoplasmic reticulum
M: Mitochondria
N: Nucleous
Nu: Nucleolus
L: Glandular lumen
EXOCYTOSIS
Identify what type of cell is shown and from what organ

EM illustrates typical protein-secreting cells from the pancreas.
What type of cell is depicted below and what process is occurring

Secretory cells of the Pancreas
Exocytosis: A cytoplasmic vesicle (secretory vesicle or granule) fuses with the plasma membrane to release its contents to the extracellular space
What is depicted in the image

The endoplasmic reticulum (ER) is an anastomosing network of intercommunicating channels or cisternae formed by a continuous membrane
- Some regions bear many ribosomes that form larger complexes (polyribosomes or polysomes) appearing rough (RER) and other regions appearing smooth (SER).
- Site for protein and lipid biosynthesis

What is depicted in the image

Electron micrograph RER in a cell specialized for protein synthesis and secretion.
What is depicted in the image

Higher magnification of RER
What is depicted in the image

RER is strongly stained purple in the cytoplasm of a nerve cell
What is depicted in the image

Electron micrograph of a nerve cell body showing the RER and free ribosomes
What is depicted in the image

Cytoplasmic basophilia in a developing red blood cell (RBC)
Cytoplasmic basophilia
It is observed in cells that produce large amounts of protein that will remain in the cell • Basophilia is caused by the presence of large amounts of RNA present in the polysomes
Nissl bodies
- The large basophilic bodies of nerve cells
- Consist of both rER and large numbers of free ribosomes
What is depicted in the image

Electron micrograph of the SER in an interstitial (Leydig) cell of the testis
Smooth Endoplasmic Reticulum(SER)
- Synthesis of phospholipids and steroids that form cell membranes
- Prominent in steroid hormone-secreting cells (e.g. adrenal cortex)
- Detoxification of lipidsoluble drugs and alcohol (e.g. prominent in hepatocytes) •Glycogen metabolism in hepatocytes
- Ca2+ storage and controlled release (e.g. in the striated muscle cell)
What is depicted in the image below

Electron micrograph shows the extensive Golgi apparatus in an islet cell of the pancreas
CGN: cis Golgi TGN: trans Golgi
What is depicted in the image

“Negative” Golgi apparatus
What is depicted int he image, what are the arrows pointing to ?

Electron micrograph shows the electron dense lysosomes (L) in a macrophage. Arrow points to the less electron-dense secondary lysosome
Lysosomes
- Major digestive compartment
- Autophagy
- Vesicles with the extracellular material taken by endocytosis fuses with a lysosome to make the secondary or heterolysosome
What is depicted in this image

H&E staining of cells with mitochondria shown in the cytoplasm as numerous eosinophilic structures (arrows)
What is depicted in the image

Mitochondria

What is depicted in the image

Peroxisomes
What is the arrow pointing to

Nucleus!
(Liver cells have large central nuclei)
Be able to label this pic


Identify the Heterochromatin and Euchromatin

Electron micrograph of a nucleus H: Heterochromatin, E: Euchromatin
Euchomatin:
– More open structure, rich with transcriptionally active genes
– Predominates in active cells (e.g. neurons)
Heterochromatin:
-More compact, with little or no transcriptional activity
– Predominates in less active cells (e.g. circulating lymphocytes)
What is depicted in the image as well as what does NP stand for

Nuclear Envelope
NP: nuclear pore
What phase of mitosis is depicted

Interphase
What phase of mitosis is depicted

Early prophase
What phase of mitosis is depicted

Late Prophase
What phase of mitosis is depicted

Metaphase
What phase of mitosis is depicted

Early and Late anaphase
What phase of mitosis is depicted

Early and late telophase and Cytokinesis
What is depicted in the image

Electron micrograph of a fibroblast cytoplasm
MT, microtubules
MF, actin filaments
Microtubules
- Maintain cell shape and rigidity
- Organize and move organelles
- Support cilia and flagella
- Participate in vesicular transport
- Separate chromosomes during the process of cell division
What is depicted in the image

Electron micrograph of a microtubules in the mitotic spindle in a dividing cell
Identify structures in the imagine


What is depicted in the image below

Electron micrograph of lipid droplets (L) in adrenal cortex cells
What is depicted in the image below

Electron micrograph of glycogen granules in cells with high metabolic activity
What is depicted in this image

Pigment deposits in liver cells