Histology Flashcards

1
Q

Preserve tissue structure and prevent degradation

A

FIXATION

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2
Q

To remove cell water and fixative by using series of Alcohol solutions

A

Dehydration

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3
Q

Alcohol is removed from the tissue using a clearing agent

A

Clearing

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4
Q

What is a popular clearing agent?

A

Xylene

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5
Q

Cleared tissue is soaked in melted paraffin wax. Also to facilitate handling and cutting of tissue

A

Infiltration

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6
Q

Paraffin-infiltrated tissue is placed in a small mold. Done to obtain a rigid consistency of the specimen for sectioning

A

Embedding

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7
Q

Paraffin block is trimmed to expose the tissue for sectioning (slicing) on a microtome

A

Trimming

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8
Q

Applying dyes on sections aids in the visualization of the architectural pattern of tissues and their physical characteristics and the relationship of cells and extracellular materials

A

Staining

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9
Q

Processes involved in preparing a slide

A

Fixation
Dehydration
Clearing
Infiltration
Embedding
Trimming
Staining

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10
Q

Most commonly used dye that turns to purplish blue

A

Hematoxylin

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11
Q

Most commonly used dye that turns to red or pink

A

Eosin

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12
Q

Type of specialised cell responsible for movement

A

Muscle and other contractile cells

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13
Q

Type of specialised cell responsible for adhesive and tight junction between cells

A

Epithelial cells

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14
Q

Type of specialised cell responsible that synthesize and secrete components of the
ECM

A

Fibroblasts, cells of bone and cartilage

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15
Q

Type of specialised cell responsible that convert physical and chemical stimuli to action potentials

A

Neurons and sensory cells

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16
Q

Type of specialised cell responsible for synthesis and secretion of glycoproteins

A

Cells of digestive glands

17
Q

Type of specialised cell responsible for synthesis and secretion of glycoproteins

A

Cell of mucous glands

18
Q

Type of specialised cell responsible for synthsis and secretion of steriods

A

Certain cells of adrenal gland, testis,ovary

19
Q

Type of specialised cell responsible for ion transport

A

Cells of Kidney and salivary gland ducts

20
Q

Type of specialised cell responsible for intracellular digestion

A

Macrophages and neutrophils

21
Q

Type of specialised cell responsible for lipid storage

22
Q

Type of specialised cell responsible for metabolic absorption

A

Cells lining the intestine

23
Q

Protein linked with sugar chains and acts as receptor for cell adhesion and cell recognition

A

Glycoprotein (under Glycocalyx)

24
Q

Lysosomes is abundant with what immune cells?

A

Macrophages and Neutrophils

25
degrade denatured or otherwise nonfunctional polypeptides; Remove proteins no longer needed by the cell; they can be detected once cannot be unfolded by ubiquitin
Proteosomes
26
intracellular digestion and turnoverof cellular components
Lysosomes
27
Mitochondria of stressed cell releases what fromthe inner membrane, triggering a regulated series of events culminating in cell death (apoptosis)
Cytochrome C
28
Type of inclusion: accumulations of lipid-filling adipocytes (fat cells)
Lipid droplets
29
Type of inclusion: aggregates of carbohydrates polymer in which glucose is stored
Glycogen granules
30
Type of inclusions: Pigmented deposits a dark brown granules
Melanin
31
Type of inclusions: complex mix of material partly derived from residual bodies after lysosomal digestion
Lipofuscin
32
Type of inclusions: dense brown aggregate of denatured ferritin proteins, results from phagocytosis of red blood cells
Hemosiderin
33
Interphase: A period which accumulate the enzyme and nucleotides REQUIRED for DNA replication
G1
34
Interphase: Period for preparation of mitosis
G2
35
Interphase: Activities may be temporarily or permanently suspended
G0
36
Phases of Mitosis
Prophase - dispersed chromosomes Metaphase - Chromosomes aligned in equatorial plane Anaphase - pulled apart Telophase - Cytokinesis
37
Function of Epithelial
Covering Absorption Glandular Secretion
38
Function of Connective Tissue
Support and protection of tissues/organs