Histology Flashcards
Preserve tissue structure and prevent degradation
FIXATION
To remove cell water and fixative by using series of Alcohol solutions
Dehydration
Alcohol is removed from the tissue using a clearing agent
Clearing
What is a popular clearing agent?
Xylene
Cleared tissue is soaked in melted paraffin wax. Also to facilitate handling and cutting of tissue
Infiltration
Paraffin-infiltrated tissue is placed in a small mold. Done to obtain a rigid consistency of the specimen for sectioning
Embedding
Paraffin block is trimmed to expose the tissue for sectioning (slicing) on a microtome
Trimming
Applying dyes on sections aids in the visualization of the architectural pattern of tissues and their physical characteristics and the relationship of cells and extracellular materials
Staining
Processes involved in preparing a slide
Fixation
Dehydration
Clearing
Infiltration
Embedding
Trimming
Staining
Most commonly used dye that turns to purplish blue
Hematoxylin
Most commonly used dye that turns to red or pink
Eosin
Type of specialised cell responsible for movement
Muscle and other contractile cells
Type of specialised cell responsible for adhesive and tight junction between cells
Epithelial cells
Type of specialised cell responsible that synthesize and secrete components of the
ECM
Fibroblasts, cells of bone and cartilage
Type of specialised cell responsible that convert physical and chemical stimuli to action potentials
Neurons and sensory cells