Histology Flashcards
1
Q
Simple squamous: Location and function?
A
- Alveoli in lungs, Lining of heart, blood vessels and lymphatic vessels
- Allows materials to pass through by diffusion and filtration
- Secretes lubricating substance
2
Q
Simple cuboidal: Location and function?
A
- In ducts and secretory portions of small glands
- Kidney tubules
- Secretes and absorbs
3
Q
Simple columnar: Location and function?
A
- Ciliated tissues – bronchi, uterine tubes and uterus
- Non-ciliated – digestive tract and bladder
- Absorbs and also secretes mucous and enzymes
4
Q
Pseudostratified columnar: Location and function
A
- Ciliated – respiratory epithelia
- Secretes mucous
- Ciliated tissue moves mucous
5
Q
Stratified squamous: Location and function?
A
- Oesophagus
- Mouth
- Vagina
- Protects against abrasion
6
Q
Stratified cuboidal: Location and function?
A
- Sweat glands
- Salivary glands
- Mammary glands
- Protective tissue
7
Q
Stratified columnar: Location and function?
A
- Male urethra
- Ducts of some glands
- Secretes and protects
8
Q
Transitional (urothelium): Location and function?
A
- Bladder
- Urethra
- Ureters
- Allows urinary organs to expand and stretch
9
Q
A
- Haematoxylin and Eosin (H&E)
- Purple: Haematoxylin – stains acidic structures e.g. nuclei, ribosomes, rough ER, DNA, RNA
- Pink: Eosin – stains basic structures e.g. proteins
10
Q
A
- Masson’s Trichrome
- Connective tissue technique
- Collagen stain
- Purple: Nuclei and basophils
- Blue: Collagen
- Red: Cytoplasm, muscle, erythrocytes, keratin
11
Q
A
- Van Gieson
- Connective tissue
- Can be combined with an elastin stain
- Purple: Nuclei
- Yellow: Erythrocytes and cytoplasm
- Red: Collagen
12
Q
A
- Alcian Blue
- Mucin stain
- Usually combined with H&E or van Gieson
- Purple: Certain types of mucin
- Blue: Certain types of mucin and van Gieson
13
Q
A
- Periodic Acid – Schiff (PAS)
- Histochemical – stains specific cellular compartments
- Pink: Complex carbohydrates
- PAS positive – mucin from goblet cells of gastro and respiratory basement membranes, brush borders of kidney tubules, small and large intestine
- Pink: Cartilage, collagen, glycogen
14
Q
A
- Nissl Methylene Blue
- Stains rough ER in neurons = Nissl substance
- Purple: Rough ER
15
Q
A
- Giemsa
- For blood cells and smears of cells, bone marrow
- Purple: Nuclei
- Blue: Background cytoplasm
- Pink: Erythrocytes
16
Q
A
- Azan
- Connective tissue, fine detail, respiratory epithelium
- Red: Nuclei
- Purple: Collagen, basement membrane, mucin
- Yellow: Muscle, erythrocytes
17
Q
A
- Osmium
- Heavy metal stain
- Stains for lipids
- Identifying myelinated neurons
18
Q
A
- Perls’ Prussian Blue
- Detect the presence of iron in biological tissues
19
Q
A
