Histological Structure of White Blood Cells

Question 1

• Have shorter life span (most last hours to days)

Answer 1

Leucocytes

Question 2

• Normally, total leucocytic count is about……………….

Answer 2

4.000-11.000/mm³.

Question 3

Granular Leucocytes

Answer 3

• Neutrophils
• Eosinophils
• Basophils

Question 4

Non-granular Leucocytes

Answer 4

• Lymphocytes

- Monocytes

Question 5

Number of Neutrophils:…………… of total leucocytic count

Answer 5

60-75%

Question 6

Neutrophils
Size :……………..
Shape:……………….
Nucleus:…………………

Answer 6

 Size : 12-15 µm
 Shape: Rounded.
 Nucleus: condensed and segmented 
(multilobed, formed of 3- 5 lobes 
connected by thin strands of chromatin)

Question 7

Life span of Neutrophils:

Answer 7

6-8 hours in blood and 1-4 days in connective tissue before dying by apoptosis.

Question 8

 Some mature neutrophils of human female show sex chromosome as a drum stick appendage (Barr body), which is the…………………….

Answer 8

inactive X-chromosome

Question 9

immature cells (band neutrophils) contain one bent 
rod (or horse-shoe) nucleus. Band neutrophils are not more than ................. in blood film under normal condition

Answer 9

1-2%

Question 10

Neutrophilic granules (80%):

Answer 10

small & numerous

• Alkaline phosphatase
• Bacteriostatic enz (lactoferrin).
• Collagenase

Question 11

Azurophilic granules in neutrophils (20%):lysosomes

Answer 11

Large& few
Hydrolytic enzymes
Proteolytic and lipolytic

Question 12

………………….. are the first line of defense against bacterial invasion

Answer 12

Neutrophils

Question 13

Eosinophils:

• number …………….
• life span ……………….

Answer 13

 Number: 1-5% of total leucocytic count.

 Life span: Few hours in blood and 1-2 weeks in connective tissue.

Question 14

 Increased number : Eosinophilia

Answer 14

(in allergy & parasitic infestation)

Question 15

Eosinophils:
Size & shape:…………………..
Nucleus: ……………………..
Cytoplasm:………………

Answer 15

 Size & shape: rounded cells, 12-15 µm in diameter.
 Nucleus: bilobed or horse-shoe
• Cytoplasm: small Golgi apparatus, few mitochondria, rER, moderate amount of glycogen. packed with numerous large, refractile and acidophilic granules.

Question 16

Specific granules in Eosinophils

Answer 16

• large ovoid

- Crystalline electron dense core

Question 17

Azurophilic granules in Eosinophils

Answer 17

Lysosomes

Question 18

Membrane bounded specific granules in Eosinophils

Answer 18

They are large ovoid with
crystalline electron dense core called the internum surrounded by
less dense zone called the externum.
These granules contain:
1- major basic proteins (MBP) and eosinophilic peroxidase (both
have anti-parasitic worms & helminths).
2- contain histaminases and arylsulphatase (anti-allergic) which
degrade histamine and slow reacting substance of anaphylaxis (SRSA) released by mast cells and basophils during hypersensitivity
reaction

Question 19

Azurophilic granules in Eosinophils

Answer 19

smaller and less in number. They are
lysosomes containing hydrolytic enzymes (anti-parasitic and also
hydrolyze the Ag-Ab complex).

Question 20

Basophils:
 Number:………………
 Life span……………………..

Answer 20

 Number: 0.5-1% of total leucocytic count.
 Life span: Few hours in blood and several months in
connective tissue.

Question 21

 Increased number: basophilia in …………………

Answer 21

allergic reactions

Question 22

Basophils:
Size & shape:…………………..
Nucleus: ……………………..
Cytoplasm:………………

Answer 22

 Size & shape: rounded cells, 10-12 µm in diameter.
 Nucleus: is irregular (or S-shape).
 Cytoplasm: is packed with large basophilic granules
masking the nucleus

Question 23

Basophils stained by:

Answer 23

(metachromatically stained by Toluidine blue).

Question 24

Specific granules in Basophils

Answer 24

• Large electron dense.
  -Contain heparin,
  histamine, ECF & NCF.

Question 25

Azurophilic granules in Basophils

Answer 25

Few lysosomes