Histological structure of RBCs Flashcards
❖Blood volume is about ………..
5 L.
Blood volume Is formed of………….plasma
55%
Blood volume Is formed of………….Blood cells
45%
Granulocytes ………….,…………,…………..,………..,
Neutrophil,
, Basophils ,
Eosinophils
Agranulocytes………..,…………….,
Lymphocyte,
Monocyte
Color of Single fresh erythrocyte
pale greenish yellow
Staining of blood film
Leishman stain
RBCs appear ………………….. with …………….. center
(normochromic)
acidophilic,
pale,
In slow circulation, RBCs adhere to each other like pile of coins
rouleaux appearance
Shape of RBCs :
❖Biconcave disc.
❖Thick periphery of 2.6 μm and thin center of 1 μm
Causes of biconcave appearance are:
- Molecular structure of the cell membrane.
2. Colloidal Hb complex inside cells
Size of RBCs :
The normal diameter is 7.5 µm (average
diameter).
Microcyte
< 6 µm
Macrocyte
> 9 µm
Abnormal shapes of RBCs :
if RBCs are put in hypertonic solution as 2% NaCl, they become shrunken (smaller with notched outline) due to loss of water
Crenation
Abnormal shapes of RBCs :
if RBCs are put in hypotonic solution as distilled water, they first swell, become spherical and then rupture leaving remnants of cell membrane called ghosts.
Spherocytosis
abnormal shape of RBCs occurring in certain diseases as in sickle cell anemia, in which cells appear like a sickle or crescent
Poikilocytosis
great percent of size of RBCs variation
Anisocytosis
Life span of erythrocytes
About 120 days
Aged cells are destroyed in ………….. , ………….. and ………….. by ………………
liver,
spleen,
bone marrow,
macrophages
Energy requirement in RBCs are met by:
- Soluble enzymes for glycolysis.
2. Hexose monophosphatase shunt which produce ATP
RBCs are surrounded by a ……………….. & ………………………
selectively permeable,
Plastic cell membrane.
Inner surface associated with cytoskeletal
protein ………………………
maintain biconcave shape
RBCs are surrounded by ………………………. cell membrane.
Most of the membrane proteins are……………………
lipoprotein,
integral membrane proteins
Outer surface is covered by ………………….
carbohydrate-rich glycocalyx
Inner surface of cell membrane is associated with
several peripheral cytoskeleton proteins e.g………………………
spectrin,
ankyrin and actin.
Size of RBCs
7-8 um
Count of RBCs
Females: 4.5-5
Males: 5-5.5 million/mm3
………… is responsible for biconcave shape
Spectrin
RBCs are flexible and can squeeze to pass through narrow blood capillaries
(plasticity)
in blood film and some diseases can form …………………
appearance
rouleaux
✓Sickle cell anemia:
abnormal Hb
✓Spherocytosis:
abnormally shaped cells
function of cytoskeleton proteins
Plasticity of the cell membrane to squeeze inside capillaries without cell injury
Highly selective cell membrane
allows exchange of gases & prevents escape of Hb
