Histological structure of RBCs

Question 1

❖Blood volume is about ………..

Answer 1

5 L.

Question 2

Blood volume Is formed of………….plasma

Answer 2

55%

Question 3

Blood volume Is formed of………….Blood cells

Answer 3

45%

Question 4

Granulocytes ………….,…………,…………..,………..,

Answer 4

Neutrophil,
, Basophils ,
Eosinophils

Question 5

Agranulocytes………..,…………….,

Answer 5

Lymphocyte,

Monocyte

Question 6

Color of Single fresh erythrocyte

Answer 6

pale greenish yellow

Question 7

Staining of blood film

Answer 7

Leishman stain

Question 8

RBCs appear ………………….. with …………….. center
(normochromic)

Answer 8

acidophilic,

pale,

Question 9

In slow circulation, RBCs adhere to each other like pile of coins

Answer 9

rouleaux appearance

Question 10

Shape of RBCs :

Answer 10

❖Biconcave disc.

❖Thick periphery of 2.6 μm and thin center of 1 μm

Question 11

Causes of biconcave appearance are:

Answer 11

1. Molecular structure of the cell membrane.

2. Colloidal Hb complex inside cells

Question 12

Size of RBCs :

Answer 12

The normal diameter is 7.5 µm (average

diameter).

Question 13

Microcyte

Answer 13

< 6 µm

Question 14

Macrocyte

Answer 14

> 9 µm

Question 15

Abnormal shapes of RBCs :
if RBCs are put in hypertonic solution as 2% NaCl, they become shrunken (smaller with notched outline) due to loss of water

Answer 15

Crenation

Question 16

Abnormal shapes of RBCs :
if RBCs are put in hypotonic solution as distilled water, they first swell, become spherical and then rupture leaving remnants of cell membrane called ghosts.

Answer 16

Spherocytosis

Question 17

abnormal shape of RBCs occurring in certain diseases as in sickle cell anemia, in which cells appear like a sickle or crescent

Answer 17

Poikilocytosis

Question 18

great percent of size of RBCs variation

Answer 18

Anisocytosis

Question 19

Life span of erythrocytes

Answer 19

About 120 days

Question 20

Aged cells are destroyed in ………….. , ………….. and ………….. by ………………

Answer 20

liver,
spleen,
bone marrow,
macrophages

Question 21

Energy requirement in RBCs are met by:

Answer 21

1. Soluble enzymes for glycolysis.

2. Hexose monophosphatase shunt which produce ATP

Question 22

RBCs are surrounded by a ……………….. & ………………………

Answer 22

selectively permeable,

Plastic cell membrane.

Question 23

Inner surface associated with cytoskeletal

protein ………………………

Answer 23

maintain biconcave shape

Question 24

RBCs are surrounded by ………………………. cell membrane.
Most of the membrane proteins are……………………

Answer 24

lipoprotein,

integral membrane proteins

Question 25

Outer surface is covered by ………………….

Answer 25

carbohydrate-rich glycocalyx

Question 26

Inner surface of cell membrane is associated with

several peripheral cytoskeleton proteins e.g………………………

Answer 26

spectrin,

ankyrin and actin.

Question 27

Size of RBCs

Answer 27

7-8 um

Question 28

Count of RBCs

Answer 28

Females: 4.5-5

Males: 5-5.5 million/mm3

Question 29

………… is responsible for biconcave shape

Answer 29

Spectrin

Question 30

RBCs are flexible and can squeeze to pass through narrow blood capillaries

Answer 30

(plasticity)

Question 31

in blood film and some diseases can form …………………
appearance

Answer 31

rouleaux

Question 32

✓Sickle cell anemia:

Answer 32

abnormal Hb

Question 33

✓Spherocytosis:

Answer 33

abnormally shaped cells

Question 34

function of cytoskeleton proteins

Answer 34

Plasticity of the cell membrane to squeeze inside capillaries without cell injury

Question 35

Highly selective cell membrane

Answer 35

allows exchange of gases & prevents escape of Hb