Histological stains Flashcards

1
Q

Fontana stain

A

+ve for melanin- Melanoma

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2
Q

Congo red

A

+ve for amyloid- Amyloidosis
apple green birefringence

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3
Q

Rhodanine stain

A

Golden brown against blue counterstain +ve for copper
– Wilson’s disease

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4
Q

Prussian blue

A

+ve for iron – Haemachromatosis

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5
Q

Perl’s stain

A

+ve for iron – Haemachromatosis

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6
Q

Cytokeratin

A

+ve for epithelial cells – Carcinoma

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7
Q

CD45

A

+ve for lymphoid cells – lymphocytes

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8
Q

Ziehl-Neelson

A

Red against a blue background
+ve for acid-fast bacilli – TB

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9
Q

Rhodamine-Auramine stain

A

Bright yellow – TB

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10
Q

Gomori’s methanamine silver stain

A

Flying saucer shaped cysts – Pneumocystic jirovecii

Candida, Aspergillus

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11
Q

Modified Kinyoung acid fast stain

A

+ve for Cryptosporidium parvum

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12
Q

India ink stain

A

Yeast cells surrounded by halos – Cryptococcus neoformans

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13
Q

Giemsa stain

A

Cystoplasmic inclusions – Chlamydisa psittaci

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14
Q

Fite stain

A

+ve for Mycobacterium leprae

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15
Q

Vimentin stain

A

Mensenchymal tissue

Sarcomas, Endometrial Carcinoma, RCC, Meningioma

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16
Q

S100

A

Schwannoma, melanoma

17
Q

Desmin

A

rhabdomyosarcoma (muscle cells)

18
Q

GFAP

A

Neuroglia

Glioblastoma, Astrocytoma, Oligodendrogliomas, Ependymomas

19
Q

Chromogranin and synaptophsyin

A

Neuroendocrine cells

Small cell lung cancer, carcinoid tumour

20
Q

Neurofilament stain

A

neuronal tumours

neuroblastomas, medulloblastomas, retinoblastomas

21
Q

Periodic acid Schiff

A

Intracytoplasmic globules of A1AT,

pneumocystitis