Histochemistry of Mammalian Tissue

Question 1

What is histology

Answer 1

studying the cellular anatomy of tissues

Question 2

When a tissue is removed from an organism what happens to the tissue?

Answer 2

It starts to decay

Question 3

What is autolysis

Answer 3

Self-destruction

Question 4

How to prevent the decaying of the tissue?

Answer 4

Its placed in a fixative

Question 5

Name the types of fixative

Answer 5

1. Alcohol(including ethanol, methanol, and isopropanol)
2. Active acid
   3 Acetone

Question 6

How do the fixatives work?

Answer 6

By precipitating proteins and nucleic acids in cells to come out and clump together

Question 7

The precipitin process can do what to the tissue?

Answer 7

damage is and create artifacts

Question 8

Are artifacts seen in living tissues

Answer 8

No, only fixed tissues

Question 9

What’s another type of fixatives?

Answer 9

crosslinking agents like formaldehyde

Question 10

Crosslinking is better because…?

Answer 10

Retains more of the natural tissues structure and produce fewer artifacts

Question 11

Human and animal tissues are usually fixed in?

Answer 11

10% neutral buffered formalin

Question 12

What sections of tissues are usually stained to make the cells visible

Answer 12

Thin sections

Question 13

Differential stains will _____?

Answer 13

React in distinct ways with various cell components based on some chemical property

Question 14

The most common differential stain for animal tissue is

Answer 14

Hematoxylin and eosin(H&E)

Question 15

Hematoxylin is a ______ dye that binds to ______ like the DNA

Answer 15

positively charged, negatively charged molecules

Question 16

Hematoxylin does what to the nucleus

Answer 16

Turns it blue

Question 17

Eosin is a ____ that binds to ______

Answer 17

negatively charged dye, positively charged molecules

Question 18

Eosin turns the cytoplasm ___

Answer 18

Pink

Question 19

How does cross linking agents like formaldehyde work?

Answer 19

By creating covalent chemical bridges within and between protein and nucleic acid molecules

Question 20

What happens after the tissue has been fixed

Answer 20

They must be prepared for sectioning

Question 21

How are thin slices of the tissue cut?

Answer 21

By using microtome

Question 22

What are the two major types of sections used in histology?

Answer 22

paraffin-embedded and frozen

Question 23

To prepare frozen sections, the tissue is placed in what?

Answer 23

cryoembedding medium

Question 24

What is a microtome mounted inside a refrigerated chamber that keeps the tissue at around -20 Celsius

Answer 24

Cryostat