Exam #2 Flashcards

1
Q

In size exclusion which has his a solid and which is a liquid

A

Stationary, Mobile

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2
Q

What is an affinity chromatography

A

A highly specific interaction between antigen-antibody or enzyme-substrate

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3
Q

What is peak fraction

A

Contains the highest concentration of the molecule

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4
Q

During immunostaining, what does permeabilization do?>

A

Partial remove the plasma membrane

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5
Q

What is epitope

A

What an antibody binds too

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6
Q

What is a antigen

A

Contains an epitope

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7
Q

Difference between direct and indirect immunostaning

A

Direct has low sensitivity since there is only one labeled antibody molecules

Indirect has a higher sensitivity there is more than one labelled antibody molecule

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8
Q

During immunostaning, why shouldn’t the sample be expose to light

A

The fluorophore on the secondary antibody will gradually weaken with exposure to light

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9
Q

What is fluorescence

A

An event in which a fluorophore temporarily captures an excitation wavelength

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10
Q

What is co-localization

A

When the structures appear on the same virtual sections

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11
Q

What is the purpose of dichroic mirror

A

To allow emission wavelength but not excitation wavelength to pass through

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12
Q

What is the purpose of emission filter

A

It increase sensitivity by allowing an emission wavelength of a desired wavelength range to pass through

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13
Q

What is the purpose for excitation filter

A

Allows the desired excitation wavelength

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14
Q

Between epifluorescence and confocal, which one requires a shorter exposure time?

A

epifluorescence

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15
Q

What is the length of an emission and excitation wavelength?

A

For the emission its: 488nm
For the excitation its: 525nm

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16
Q

Between the emission filter and the excitation filter, which one is between the specimen and the eyepiece or camera

A

emission filter

17
Q

Who process and prepare tissue samples and who are responsible for looking at them?

A

Histotechs prepare the tissue samples and pathologist are responsible for looking at them

18
Q

In histology, what’s the purpose of fixative?

A

To prevent the tissue for degrading

19
Q

What fixative can be used for precipitating

A

Alcohol, Acetic acid, acetone

20
Q

In preparation for histology, prepared blocks of tissue are sliced into think sections using an instrument called a _____

21
Q

Why must CitricSolV be kept in the fume hood

A

Can irritate the skin and lungs when inhaled

22
Q

What the difference between serum and plasma?

A

Serum lacks fibrinogen
Plama carries the major electrolytes ions sodium

23
Q

What are some negative effect that refrigeration can have on blood samples

A

-Causes the blood to clump together
-Forms lysed cells

24
Q

The two common techniques used in blood analysis are?

A

Differential blood counts and Whole blood smears

25
What are the five major types of white blood cells that are measured by differential blood count
1. Neutrophil 2. Eosinophil 3. Basophil 4. Lymphocyte 5. Monocyte
26
The faster the spreader slide
The longer and thinner the film will be
27
The slower the spreader slide
The shorter and thicker the film will be
28
If the angle is greater than 30 degrees then the smear will be
thick
29
If the angle is less than 30 degrees then the smear will be
thin
30
When blood viscosity increases then
A thinner smear is needed
31
When blood viscosity decreases then
A thicker smear is needed