Exam #2

Question 1

In size exclusion which has his a solid and which is a liquid

Answer 1

Stationary, Mobile

Question 2

What is an affinity chromatography

Answer 2

A highly specific interaction between antigen-antibody or enzyme-substrate

Question 3

What is peak fraction

Answer 3

Contains the highest concentration of the molecule

Question 4

During immunostaining, what does permeabilization do?>

Answer 4

Partial remove the plasma membrane

Question 5

What is epitope

Answer 5

What an antibody binds too

Question 6

What is a antigen

Answer 6

Contains an epitope

Question 7

Difference between direct and indirect immunostaning

Answer 7

Direct has low sensitivity since there is only one labeled antibody molecules

Indirect has a higher sensitivity there is more than one labelled antibody molecule

Question 8

During immunostaning, why shouldn’t the sample be expose to light

Answer 8

The fluorophore on the secondary antibody will gradually weaken with exposure to light

Question 9

What is fluorescence

Answer 9

An event in which a fluorophore temporarily captures an excitation wavelength

Question 10

What is co-localization

Answer 10

When the structures appear on the same virtual sections

Question 11

What is the purpose of dichroic mirror

Answer 11

To allow emission wavelength but not excitation wavelength to pass through

Question 12

What is the purpose of emission filter

Answer 12

It increase sensitivity by allowing an emission wavelength of a desired wavelength range to pass through

Question 13

What is the purpose for excitation filter

Answer 13

Allows the desired excitation wavelength

Question 14

Between epifluorescence and confocal, which one requires a shorter exposure time?

Answer 14

epifluorescence

Question 15

What is the length of an emission and excitation wavelength?

Answer 15

For the emission its: 488nm
For the excitation its: 525nm

Question 16

Between the emission filter and the excitation filter, which one is between the specimen and the eyepiece or camera

Answer 16

emission filter

Question 17

Who process and prepare tissue samples and who are responsible for looking at them?

Answer 17

Histotechs prepare the tissue samples and pathologist are responsible for looking at them

Question 18

In histology, what’s the purpose of fixative?

Answer 18

To prevent the tissue for degrading

Question 19

What fixative can be used for precipitating

Answer 19

Alcohol, Acetic acid, acetone

Question 20

In preparation for histology, prepared blocks of tissue are sliced into think sections using an instrument called a _____

Answer 20

microtome

Question 21

Why must CitricSolV be kept in the fume hood

Answer 21

Can irritate the skin and lungs when inhaled

Question 22

What the difference between serum and plasma?

Answer 22

Serum lacks fibrinogen
Plama carries the major electrolytes ions sodium

Question 23

What are some negative effect that refrigeration can have on blood samples

Answer 23

-Causes the blood to clump together
-Forms lysed cells

Question 24

The two common techniques used in blood analysis are?

Answer 24

Differential blood counts and Whole blood smears

Question 25

What are the five major types of white blood cells that are measured by differential blood count

Answer 25

1. Neutrophil
2. Eosinophil
3. Basophil
4. Lymphocyte
5. Monocyte

Question 26

The faster the spreader slide

Answer 26

The longer and thinner the film will be

Question 27

The slower the spreader slide

Answer 27

The shorter and thicker the film will be

Question 28

If the angle is greater than 30 degrees then the smear will be

Answer 28

thick

Question 29

If the angle is less than 30 degrees then the smear will be

Answer 29

thin

Question 30

When blood viscosity increases then

Answer 30

A thinner smear is needed

Question 31

When blood viscosity decreases then

Answer 31

A thicker smear is needed