Exam #2 Flashcards
In size exclusion which has his a solid and which is a liquid
Stationary, Mobile
What is an affinity chromatography
A highly specific interaction between antigen-antibody or enzyme-substrate
What is peak fraction
Contains the highest concentration of the molecule
During immunostaining, what does permeabilization do?>
Partial remove the plasma membrane
What is epitope
What an antibody binds too
What is a antigen
Contains an epitope
Difference between direct and indirect immunostaning
Direct has low sensitivity since there is only one labeled antibody molecules
Indirect has a higher sensitivity there is more than one labelled antibody molecule
During immunostaning, why shouldn’t the sample be expose to light
The fluorophore on the secondary antibody will gradually weaken with exposure to light
What is fluorescence
An event in which a fluorophore temporarily captures an excitation wavelength
What is co-localization
When the structures appear on the same virtual sections
What is the purpose of dichroic mirror
To allow emission wavelength but not excitation wavelength to pass through
What is the purpose of emission filter
It increase sensitivity by allowing an emission wavelength of a desired wavelength range to pass through
What is the purpose for excitation filter
Allows the desired excitation wavelength
Between epifluorescence and confocal, which one requires a shorter exposure time?
epifluorescence
What is the length of an emission and excitation wavelength?
For the emission its: 488nm
For the excitation its: 525nm
Between the emission filter and the excitation filter, which one is between the specimen and the eyepiece or camera
emission filter
Who process and prepare tissue samples and who are responsible for looking at them?
Histotechs prepare the tissue samples and pathologist are responsible for looking at them
In histology, what’s the purpose of fixative?
To prevent the tissue for degrading
What fixative can be used for precipitating
Alcohol, Acetic acid, acetone
In preparation for histology, prepared blocks of tissue are sliced into think sections using an instrument called a _____
microtome
Why must CitricSolV be kept in the fume hood
Can irritate the skin and lungs when inhaled
What the difference between serum and plasma?
Serum lacks fibrinogen
Plama carries the major electrolytes ions sodium
What are some negative effect that refrigeration can have on blood samples
-Causes the blood to clump together
-Forms lysed cells
The two common techniques used in blood analysis are?
Differential blood counts and Whole blood smears
What are the five major types of white blood cells that are measured by differential blood count
- Neutrophil
- Eosinophil
- Basophil
- Lymphocyte
- Monocyte
The faster the spreader slide
The longer and thinner the film will be
The slower the spreader slide
The shorter and thicker the film will be
If the angle is greater than 30 degrees then the smear will be
thick
If the angle is less than 30 degrees then the smear will be
thin
When blood viscosity increases then
A thinner smear is needed
When blood viscosity decreases then
A thicker smear is needed
