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Final Na 2 > HIS2; > Flashcards

HIS2; Flashcards

1
Q
  1. To excellently fixed intercalated disk of cardiac muscles, which
    of the following may be used?
    a. Hollande’s
    b. Helly’s
    c. Lillie’s B5
    d. Orth’s Fluid
A

b. Helly’s

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2
Q
  1. Formol sublimate fixes
    1 Neutral fats
    2 Phospholipids
    3 Lipids
    4 Elastic Fibers

a. 1&3
b. 2&4
c. 1, 2, &3
d. 1, 2, 3, &4

A

c. 1, 2, &3

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3
Q
  1. Another aldehyde which has been introduced a mixture of
    glutaraldehyde or formaldehyde

a. Zinc sulfate
b. Glyoxal
c. Acrolein
d. Michel’s solution

A

c. Acrolein

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4
Q
  1. Which of the following factors that aside from prolonging
    fixation process can also inactivate enzymes

a. Presence of mucus
b. Cold temperature
c. Presence of blood
d. Presence of fats

A

b. Cold temperature

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5
Q
  1. Which of the following is both a nuclear and histochemical
    fixative?

a. Newcomer’s
b. Acetone
c. Formol saline
d. Bouin’s

A

a. Newcomer’s

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6
Q
  1. From the choices in #45, which is both microanatomical and
    histochemical?

a. Newcomer’s
b. Acetone
c. Formol saline
d. Bouin’s

A

c. Formol saline

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7
Q
  1. What may be added to formaldehyde to prevent decomposition to formic acid?

a. Ethyl alcohol
b. Sodium thiosulfate
c. Lithium carbonate
d. Methanol

A

d. Methanol

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8
Q
  1. A formol mercuric chloride solution used for post mortem
    tissues

a. Gendre’s fixative
b. Fleming’s solution
c. Formol corrosive
d. Zenker formol

A

c. Formol corrosive

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9
Q
  1. The following fixative has a pH of more than 4.6 except:

a. Bouin’s
b. Helly’s
c. Moller’s
d. Orth’s

A

a. Bouin’s

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10
Q
  1. Which of the following fixatives that when used will inhibit
    hematoxylin?

a. Bouin’s
b. Newcomer’s
c. Alcoholic formalin
d. Osmic acid

A

d. Osmic acid

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11
Q
  1. Which of the following specimens may require immersion in 4% phenol for 3 days?

1 Uterine
2 Hyperkeratotic skin
3 Cervix
4 Fibroids

a. 1&3
b. 2&4
c. 1,2,3
d. 1,2,3&4

A

d. 1,2,3&4

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12
Q
  1. In general, which group of chemical fixatives may cause
    glycogen polarization?

a. Heat fixation
b. Aldehyde fixation
c. Picric acid fixation (Correct: Alcoholic Fixatives)
d. Mercuric chloride fixation

A

c. Picric acid fixation (Correct: Alcoholic Fixatives)

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13
Q
  1. Which of the ff. is NOT a commonly used fixative for nucleic
    acid

a. Ethanol
b. Isopropyl alcohol
c. Carnoy’s
d. Methanol

A

b. Isopropyl alcoho

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14
Q
  1. Which is the suitable fixation temperature for histochemistry?

a. 0-40 degC
b. 40 degC
c. Room temp
d. 0-4 degC

A

d. 0-4 degC

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15
Q
  1. Which of the following concentrations of glutaraldehyde have
    been found to be an ideal concentration for Immune Electron
    Microscopy?

a. 3%
b. 2.5%
c. 0.25%
d. 4%

A

c. 0.25%

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16
Q
  1. Using choices in #55, which concentration of glutaraldehyde is
    suited for needle biopsies

a. 3%
b. 2.5% – typo??
c. 0.25%
d. 4%

A

b. 2.5% – typo??

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17
Q
  1. Which of the following is recommended for fixation of CNS
    tissues & general post mortem tissues for histochemical
    examination

a. 10% Neutral buffered formalin
b. 10% formol saline
c. Formol corrosive
d. Gendre’s

A

b. 10% formol saline

18
Q
  1. From the choices in #57, which can be used to fix sputum?

a. 10% Neutral buffered formalin
b. 10% formol saline
c. Formol corrosive
d. Gendre’s

A

d. Gendre’s

19
Q
  1. Fixation temperature using autotechnicon

a. 0-40 degC
b. 40 degC
c. Room temp
d. 0-4 degC

A

b. 40 degC

20
Q
  1. The fixative of choice for preserving cell details in tissue
    photography

a. Heat fixation
b. Aldehyde fixation
c. Picric acid fixation
d. Mercuric chloride fixation

A

d. Mercuric chloride fixation

21
Q
  1. What may be added to 10-15% formalin to prevent acid
    reactions due to formic acid formation

a. Calcium carbonate
b. 10% methanol
c. Lithium carbonate
d. Magnesium hydroxide

A

a. Calcium carbonate

22
Q
  1. Which of the following may be added to prevent precipitation
    of formaldehyde to white paraformaldehyde?

a. 10% ethanol
b. 10% methanol
c. 10% butanol
d. 10% isopropanol

A

b. 10% methanol

23
Q
  1. Temperature range for decalcification

a. 37-56 degC
b. 30-37 degC
c. 18-20 degC
d. 18-30 degC

A

d. 18-30 degC

24
Q
  1. Bone decalcification for enzyme histochemistry may be
    satisfactorily achieved by using which of the following decalcifier?

a. 5-10% nitric acid
b. Phloroglucin nitric
c. EDTA
d. Von Ebner’s

A

c. EDTA

25
Q
  1. Which of the following may be added to alcohol bath in
    dehydration process to soften hard tissues

a. Phenol
b. HC
c. Perenyi’s fluid
d. Both A and C

A

a. Phenol

26
Q
  1. Preferred clearing agent for cytological studies

a. Clove oil
b. Aniline oil
c. Castor oil
d. Cedarwood oil

A

d. Cedarwood oil

27
Q
  1. Infiltrating method suited for enzyme studies

a. Celloidin
b. Gelatin
c. Paraffin
d. Plastic resins

A

b. Gelatin

28
Q
  1. From the choices in #67, which is suited for specimens with
    large and hollow cavities that tends to collapse

a. Celloidin
b. Gelatin
c. Paraffin
d. Plastic resins

A

a. Celloidin

29
Q
  1. Must be done to achieve adequate fixation
    1 Use of hypotonic fixative
    2 Agitation
    3 10:1 ratio of fixative to tissue

A. 1&2
B. 1&3
C. 2&3
D. 1,2,3
E. None
AB. 1 Only

A

E. None

30
Q
  1. Can be used to fix brain tissues for rabies diagnosis
  2. Osmic acid
  3. Carnoy’s
  4. Acetone

A. 1&2
B. 1&3
C. 2&3
D. 1,2,3
E. None
AB. 1 Only

A

C. 2&3

31
Q
  1. Can be both fixative and decalcifying agent
  2. Acetone (fixative & dehydrating)
  3. Ethyl alcohol
  4. Methyl alcohol

A. 1&2
B. 1&3
C. 2&3
D. 1,2,3
E. None
AB. 1 Only

A

E. None

32
Q
  1. Fixatives that will allow excellent trichrome
  2. Zenker’s
  3. Orth;s
  4. Moller’s

A. 1&2
B. 1&3
C. 2&3
D. 1,2,3
E. None
AB. 1 Only

A

AB. 1 Only

33
Q
  1. Nuclear and Microanatomical
  2. Helly’s (pede din sya both)
  3. Formol saline (Nucleoproteins & Microanatomical)
  4. Acetone (not a microanatomical fixative)

A. 1&2
B. 1&3
C. 2&3
D. 1,2,3
E. None
AB. 1 Only

A

A. 1&2

34
Q
  1. Embedding mold that when used will yield perfect blocks even
    without trimming

a. Leuckhart’s
b. Compound embedding unit
c. Peel away mold
d. Plastic ice trays

A

c. Peel away mold

35
Q
  1. Plastic resin suited for undecalcified bone

a. MMA
b. GMA
c. Spurr
d. Polyester

A

a. MMA

36
Q
  1. Paraffin wax substitute suited for enzyme studies

a. Ester wax
b. Carbowax
c. Paraplast

A

b. Carbowax

37
Q
  1. The most common error in histotechnology is

a. Use of concentrated solutions
b. Prolonging fixation time
c. Cutting tissues more than 5 mm thick
d. Prolonging the contact time of fixative to tissues
e. None

A

e. None

38
Q
  1. The fixative that can provide improved results in
    immunohistochemistry

a. Clarke’s solution
b. Formol acetic alcohol
c. Michel solution
d. Zinc formalin

A

d. Zinc formalin

39
Q
  1. A traditionally alcohol containing fixative used for frozen
    sections and smears

a. Carnoy’s
b. Clarke’s solution
c. Formol acetic alcohol
d. None of these

A

b. Clarke’s solution

40
Q
  1. Which of the following reagent is commonly used in
    de-zenkerization

a. Alcohol iodine
b. Saturated iodine solution
c. 70% ethanol
d. Saturated picric acid

A

a. Alcohol iodine

Final Na 2 (4 decks)

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