Hemolysis

Question 1

Define: hemolysis

Answer 1

Hemolysis: release of hemoglobin into the fluid portion of the blood as the result of breaking apart the cell walls of red blood cells

Question 2

List 2 ways hemolysis may occur. (2)

Answer 2

1. Mechanical means

2. Result of a disease or physical disorder (autohemolysis)

Question 3

Which of the 2 reasons for hemolysis is most likely? (1)

Answer 3

Mechanical means - incorrect blood drawing or specimen processing techniques

Question 4

Why should one avoid causing hemolysis?

Answer 4

May adversely affect many laboratory results

Question 5

List 6 ways to avoid mechanical hemolysis. (6)

Answer 5

1. When drawing with a syringe, do not draw back on the plunger forcefully
2. Use the appropriately-sized needle for the vein
3. If a syringe is used and blood is injected into an evacuated tube, use a 21 gauge needle and NEVER force the blood into the tube by pushing the plunger (allow the vacuum to draw the blood from the syringe)
4. When mixing blood with additives in the tubes, invert the tubes about 5 times
5. Use tubes that are at room temperature
6. Allow the antiseptic to dry before inserting the needle