Hematology: CBC and Differential

Question 1

Manual Hematocytometer Count

Answer 1

cells counted x dilution factor / total volume of cells counted

Where total volume = # primary squares x depth. and depth is 0.1 (WBC) and 0.04 (RBC)

Question 2

RBC

Answer 2

4-6 x 10⁶/uL (or x 10¹²/L)

Question 3

Platelets

Answer 3

150-450 x 10³/uL (or x10⁹/L)

Question 4

Hct (normal range)

Answer 4

35-50%

Question 5

nRBC (normal range)

Answer 5

0 nRBC/100 WBC

Question 6

Corrected WBC count when >10 nRBC are present

Answer 6

Corrected WBC = uncorrected WBC count * 100 / (100 + # of nRBCS per 100 WBC)

Question 7

WBC (Total)

Answer 7

3.6 - 10.6 x 10³/uL (or x10⁹/L)

Question 8

Neutrophils (absolute and relative)

Answer 8

1.75 - 7.5 x 10³/uL (or x10⁹/L)
50-70%

Question 9

Lymphocytes (absolute and relative)

Answer 9

1.0 - 3.2 x 10³/uL (or x10⁹/L)
18-42%

Question 10

Monocytes (absolute and relative)

Answer 10

0.1 - 1.3 x 10³/uL (or x10⁹/L)
2-11%

Question 11

Eosinophils (absolute and relative)

Answer 11

0 - 0.3 x 10³/uL (or x10⁹/L)
1-3%

Question 12

Basophils (absolute and relative)

Answer 12

0 - 0.2 x 10³/uL (or x10⁹/L)
0-2%

Question 13

Hgb (normal range)

Answer 13

12-18 g/dL

Question 14

Reticulocytes (absolute and relative)

Answer 14

20-115 x 10³/uL (or x10⁹/L)
0.5 - 2.5%

Question 15

RDW (%)

Answer 15

11.5-14.5%