Hematology

Question 0

What type of dye is used in a reticulocyte count?

Answer 0

Methylene Blue

Question 1

A reticulocyte count is a measurement of what kind of response?

Answer 1

A regenerative response

Question 2

How are reticulocyte a different from normal rbc?

Answer 2

They have dark spots or lines in them .

Question 3

What is the ratio of reticulocyte to rbc?

Answer 3

reticulocytes/1000 rbc

Question 4

Cats have how many types of reticulocytes?

Answer 4

2

Question 5

What are the two types of cat reticulocytes?

Answer 5

Punctate and Aggregate

Question 6

Which of the cat reticulocytes are the ones you count in a reticulocyte count?

Answer 6

Only the aggregate ones.

Question 7

Describe punctate reticulocytes.

Answer 7

They have 2-8 basophilic stipples.

Question 8

Describe aggregate reticulocytes.

Answer 8

They have basophilic lines - more like the typical reticulocytes.

Question 9

What does the CBC machine lyses?

Answer 9

Everything without a nucleus. (Mature RBC, platelets)

Question 10

When will a CBC machine count a RBC as a WBC?

Answer 10

If the RBC has a nucleus, it is not lyses, therefore it is counted as a WBC (artificially elevating WBC count).

Question 11

When would we know if a blood sample has N-RBCs?

Answer 11

When a differential is performed.

Question 12

How are N-RBCs seen on a differential recorded?

Answer 12

N-RBC/100 WBC

Question 13

How must a differential be corrected if N-RBCs are found on it?

Answer 13

Observed WBC count x 100 ——————————— 100 + N-RBC

Question 14

When do fibrinogen levels increase?

Answer 14

During inflammation.

Question 15

What is something that can be done in order to measure fibrinogen levels?

Answer 15

By heating plasma to 56C.

Question 16

What happens to plasma at 56C?

Answer 16

Fibrinogen pellets out, but other plasma proteins stay suspended.

Question 17

What the procedure for testing fibrinogen?

Answer 17

1. Spin two hematocrit tubes.
2. Measure TP with one of the hematocrit tubes.
3. Incubate the other hematocrit in a 56C water bath for 5 minutes.
4. Respin heated hematocrit and measure TP.
5. Figure the fibrinogen level by subtracting the second TP from the first TP. Record as mg/dl.

Question 18

What is crossmatching used for?

Answer 18

To determine compatibility for blood transfusions.

Question 19

What indicates incompatibility?

Answer 19

Agglutination (RBC clumping)

Question 20

How can you blood type cats?

Answer 20

Via cards similar to humans.

Question 21

How many blood types do dogs have?

Answer 21

11 types of blood.

Question 22

What is the difference between a major and minor crossmatch?

Answer 22

Major: Recipient serum is tested against donor packed cells to determine if the recipient has preformed antibodies against any antigens on the donor’s cells.

Minor: Recipient RBCs are tested against donor serum to detect donor antibodies directed against patient’s antigens.

Question 23

What causes a hematoma?

Answer 23

Needle has passed through vein.
Bevel of needle is only partially in vein.
Insufficient pressure on venipuncture site after needle is withdrawn.

Question 24

Lavender Top Tube (LTT)

Answer 24

EDTA-hematology

Question 25

Red Top Tube (RTT)

Answer 25

No anticoagulant

Serum

Question 26

Green Top Tube (GTT)

Answer 26

Heparin - plasma

Question 27

Serum Separator Tube (SST)

Answer 27

No anticoagulant

Gel to separate serum

Question 28

Blue Top Tube (BTT)

Answer 28

Sodium citrate - blood clotting

Question 29

Gray Top Tube (GTT)

Answer 29

Sodium Fluoride

Blood Glucose