Hematology Flashcards
Do we perform differentials on Buffy coat?
No
What is the heparin tube used for in hematology?
osmotic fragility, LAP score
What is the ME ratio?
2:1 to 5:1 - lymphs not included
What is the lifespan of platelets?
5-10 days
How many platelets are there per field?
8-10
What is Hbg F made out of?
2 alpha and 2 gamma
What does Hbg F change to?
Hb A
What is Hbg A made out of?
2 alpha and 2 beta
What is Hbg A2 made out of?
2 alpha and 2 delta
What is carboxyhemoglobin?
Carbon monoxide bound with hemoglobin
What is methhemoglobin?
When iron goes from ferrous to ferric state
Is carboxyhemoglobin/methemoglobin reversible?
Yes
What is the Drabkin’s solution used for?
Measure hemoglobin colormetrically
When do we correct WBC’s with the formula?
> 10 nRBC
What is the correction formula for WBCs?
(#WBC counted/#WBC + nRBC counted) x WBC of the instrument
What is the DF for a manual RBC count?
200
When do we use a Neubauer hemocytometer?
Count high # of WBCs and PLTs (depth: 0.1 mm)
When do we use the Fuchs hemocytometer?
Count low # eosinophils and body fluids
What makes up the Wrights stain?
methylene blue, eosin, methanol, giemsa
What stain is required for a bone marrow sample?
Perls Prussian Blue for hemosiderin (form iron takes in the bone marrow)
Where is the main storage site for iron?
Bone marrow
What is the secondary storage site for iron?
liver
What stain is used for reticulocytes?
Supravital stain (ex: new methylene blue)
What objective do we use for retics?
oil immersion
Which of the following actions may cause a body fluid cytosine preparation to have a cell button that is too thick?
a. Sample vortexes prior to slide preparation
b. Sample stored at 22C for many hours
c. Sample not mixed prior to slide preparation
d. Sample diluted prior to slide preparation
C
Which of the following morphological findings correlate with the chronic blood loss?
Micro/hypo
Which of the following anemias correlates with the given results?
RBC 3.30
Hgb 110 g/L
HCT 0.298
a. Aplastic
b. Hereditary spherocytosis
c. Hereditary elliptocytosis
d. Thalassemia
B
Aspirin blocks plt plug formation by interfering with what process?
A. Secretion
B. Shape change
C. Aggregation
D. Adhesion
C
Heparin antagonist that will remove heparin from blood samples collected from patients on heparin therapy:
Protamine sulphate
Which enzyme is responsible for fibrinolysis in vivo?
A. Coagulase
B. Plasmin
C. Fibrinolase
D. Thrombin
B
What is defective glycoprotein IIb/IIIa associated with?
Glanzmann thrombasthenia
In polycythemia vera, the platelet count is:
A. Elevated
B. Decreased
C. Normal
D. Variable
A
What causes a decrease in osmotic fragility?
Sickle cell anemia due to increased SA: V ratio (less fragile and more deformable)
Used to confirm diagnosis of activated protein C resistance:
Factor V Leiden mutation assay
