Hematocrit Value Or Packed Cell Volme

Question 1

Appratus

Answer 1

Capillary tube heparinised
Centrifuge

Question 2

Materials

Answer 2

Heaprinised tube and blood till half or three fourth of tube
Centrifuge
Scale

Question 3

Steps

Answer 3

Prick thumb by lancet
Close one end of tube by wax
Centrifuge for 5 minutes
Read pcv against scale where rbcs at level of 0 while plasma at other level

Question 4

Principle

Answer 4

Cells are separated from plasma by specific gravity
Rbcs 1090,
Wbcs and platelets or puffy coat 1060,
Plasma 1030 so bottom layer forming then puffy coat middle then rbcs at top coat

Question 5

Values

Answer 5

Males are 46% +- 5%
Females are 42% +- 5%

Question 6

Variations

Answer 6

High pcv due to polycythemia
Dehydration by vomiting, diarrhea and profuse sweating
Venous blood
Low pcv due to anemia
Over hydration or pregnancy
Arterial blood

Question 7

Why hematocrit value higher in venous blood

Answer 7

Due to chloride shift

Question 8

Pcv in high altitudes

Answer 8

Hypoxia cause erythropoietin formation from kidney
So increased rbcs production so higher pcv

Question 9

Pcv Microcytic hypochromic anemia

Answer 9

Decrease

Question 10

Heparin tube

Answer 10

Red colored