Erythrocyte Sedemntation Rate Experiment

Question 1

Appratus used

Answer 1

Westergren tube

Question 2

Clinical significance of experiment

Answer 2

Prognostic not diagnostic used for a follow up

Question 3

Materials used

Answer 3

0.5 ml of 3.8% of sodium citrate anticoagulant
2 ml of blood so ratio is
1:4
Westergren tube

Question 4

Steps

Answer 4

Add na citrate to blood and leave in tube and read plasma level after first and second hours

Question 5

Principle of the experiment

Answer 5

Rbcs settle down and form the rouleaux appearance as sodium citrate cancel ca++ charges
In body plasma protein levels neutralize electrostatic charges on rbcs so decrease repulsion forces of rbcs so they adhere together
Rbcs settle down due to their 1060 specific gravity

Question 6

Normal levels of experiment

Answer 6

In males: 5 & 8
In females: 10 & 16

Question 7

Physiological and pathological variation in results

Answer 7

See can increase physiologically in pregnant and menstruating females
Pathologically it can increase in infections, inflammation, malignant tumor present in the body, autoimmune disease and anemia
Pathological wise it can decrease in afibrinogenemia & polycythemia

Question 8

Why in inflammation the esr increase

Answer 8

Due to increase levels of plasma proteins so neutralize hydrostatic charges in rbcs so decrease their repulsion forces so adhere together

Question 9

Plasma proteins involved in neutralizing charges

Answer 9

Fibrinogen & globulin

Question 10

Westergren tube

Answer 10

Graded from upwards downwards from 0 and increasing till 200 ml