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Hematology 1 (Laboratory) > Hematocrit Determination (Worksheet #5 | M) > Flashcards

Hematocrit Determination (Worksheet #5 | M) Flashcards

1
Q

What is hematocrit (what is its component)?

A

It is the volume of packed red blood cells (PRBCs) after the centrifugation of a blood sx

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2
Q

What is the other term for Hct?

A

Packed cell volume (PCV) and erythrocyte volume fraction (EVF)

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3
Q

How is Hct reported (what are the units used)?

A

1) Percent (%)
2) Cell volume percent (CV%)
3) Volume percent (vol%)

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4
Q

What are the contributions (and advantages) of Hct determination?

A 
It is one of the:
1) simplest
2) most accurate and 
3) valuable tests 
in hematological investigation
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5
Q

What is more useful than RBC count when it comes to detecting cases of anemia?

A

Hct determination

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6
Q

What are the values (or components) needed for the manual computation of RBC indices?

A

From the values of:

1) RBC count
2) Hemoglobin level
3) Hematocrit level

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7
Q

What are the 2 methods of Hct determination?

A

1) Clay Adam’s Microhematocrit Method

2) Wintrobe Method (Macrohematocrit Method)

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8
Q

What are the materials needed for Clay Adam’s method?

A

1) EDTA blood
2) Capillary tube (blue or red)
3) Sealing clay
4) Microhct centrifuge
5) Microhct reader
6) Disposable sterile blood lancet

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9
Q

What is the process (or steps) of Clay Adam’s method?

A

1) Fill around 3/4 of the capillary tube w/ blood
2) Seal one end of the capillary tube w/ sealing clay (about 3 mm)
3) Centrifuge the blood at 10,000 rpm for 4 - 5 mins using a microhct centrifuge
4) Determine the lvl of PRBCs using a microhct reader

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10
Q

What are the characteristics of red capillary tube (used in Clay Adam’s method)?

A

1) It is heparinized

2) It has a red colored ring at the top of the capillary tube

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11
Q

What is the purpose of red capillary tube (in Clay Adam’s method)?

A

It is heparinized which is responsible for the anticoagulation of blood

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12
Q

What are the characteristics of blue capillary tube (used in Clay Adam’s method)?

A

1) It is non-heparinized

2) It has a blue colored ring at the top of the capillary tube

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13
Q

What is the purpose of blue capillary tube (in Clay Adam’s method)?

A

It is non-heparinized to not provide contribution to the anticoagulant properties of EDTA tube

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14
Q

What is the depth of sealing to the capillary tube (for Clay Adam’s method)?

A

about 3 mm

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15
Q

What is the rpm for centrifuging blood using a microhct centrifuge?

A

10,000 rpm

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16
Q

What is the time for centrifuging blood using a microhct centrifuge?

A

4 - 5 mins

17
Q

What is used to determine the lvl of PRBCs?

A

Microhct reader

18
Q

What is utilized in Wintrobe method?

A

This method utilizes a wintrobe tube

19
Q

How many are the calibrations of a Wintrobe tube (used in Wintrobe method)?

A

2

20
Q

What are the 2 types of calibrations of Wintrobe tube?

A

1) 0 to 10
2) 10 to 0
* upwards

21
Q

What is the purpose of Wintrobe tube having 0 to 10 calibration?

A

It is used for ESR

22
Q

What is the purpose of a Wintrobe tube having a 10 to 0 calibration?

A

It is used for Hct

23
Q

What is the anticoagulant of choice for Wintrobe method?

A

Double oxalate

24
Q

What is the process (or steps) of Wintrobe method?

A

1) Fill the Wintrobe tube w/ blood using a Pasteur pipette
2) Centrifuge the tube at 3,000 rpm for 30 mins
3) Read the volume of PRBCs
4) Compute for the Hct lvl using the formula

25
Q

What should be used to fill the Wintrobe tube w/ blood (in Wintrobe method)?

A

Pasteur pipette

26
Q

What is the rpm in centrifuging the tube (in Wintrobe method)?

A

3,000 rpm

27
Q

What is the time duration for centrifugation of tube (in Wintrobe method)?

A

30 mins

28
Q

What is the formula used for computing the Hct lvl (in Wintrobe method)?

A

Hematocrit (%) = height of PRBCs / height of whole blood used X 100

29
Q

What are the normal values (in CU) of Hct for male and female?

A

1) Male: 40 - 54 %

2) Female: 35 - 49 %

30
Q

What are the normal values (in SI) of Hct for male and female?

A

1) Male: 0.40 - 0.54 L/L

2) Female: 0.35 - 0.49 L/L

31
Q

What is the conversion factor for computing the normal values of Hct?

A

0.01

Hematology 1 (Laboratory) (9 decks)

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