Differential White Blood Cell Count (Worksheet #3 | P)

Question 1

What is the principle of diff WBC count?

Answer 1

It is the determination of the % of each type of WBCs in the peripheral blood

Question 2

What is peripheral blood?

Answer 2

It is the blood that is currently in the circulation

Question 3

What are the mats needed for diff WBC count?

Answer 3

1) Blood smear
2) Methanol
3) Eosin
4) Methylene Blue
5) Compound Light Microscope
6) Buffer solution pH 7.2 / Aged distilled H20
7) Differential counter
8) Cedarwood oil
9) Xylol
10) Xylol-alcohol

Question 4

What should be done first (to slide containing sx) before doing diff WBC count?

Answer 4

Staining

Question 5

What is the term used for the staining process done prior to diff WBC count?

Answer 5

Staining Jar or “Dip” Method

Question 6

What is the process of Staining Jar or “Dip” method?

Answer 6

1) Dip in solution 1 (methanol, the fixative) for 30 secs
2) Dip in solution 2 (eosin, the acidic dye) for 6 secs
3) Dip in solution 3 (methylene blue, the basic dye) for 4 secs
4) Dip in buffer solution / aged distilled H2O for 45 secs

Question 7

What is the process of diff counting?

Answer 7

1) Prep stained blood smear
2) Place one drop of cedarwood oil on the feathery edge of the stained blood smear
3) Examine the smear using LPO of the microscope. Focus on the area where the RBCs are not too overlapping or too scanty
4) Shift to OIO. Using the differential method, count 100 WBCs while differentiating them

Question 8

Explain the principle of strip differential method

Answer 8

Direction of scanning: towards up - right - down - right - up
*direction of scanning depends on the strat of MT

Question 9

How many are the different types of WBC?

Answer 9

5

Question 10

What are the different types of WBC?

Answer 10

1) Neutrophil
a. Neutrophil band (Stab cell)
b. Neutrophil segmenter
2) Lymphocyte
3) Monocyte
4) Eosinophil
5) Basophil

Question 11

What are the characteristics of neutrophil band?

Answer 11

1) It is the younger form of neutrophil

2) It has a C, S, U, or horseshoe-shaped nucleus

Question 12

What are the characteristics of neutrophil segmenter?

Answer 12

1) Its nucleus is broken into segments

2) Its cytoplasm contains small, pinkish granules

Question 13

What are the characteristics of lymphocyte?

Answer 13

1) Its nucleus is compact and usually round

2) Cytoplasm is light blue and scanty

Question 14

What are the characteristics of monocyte?

Answer 14

1) Its nucleus is spongy and sprawling w/ brain-like convolutions
2) Its cytoplasm is color gray
3) Vacuoles are sometimes present (in the cytoplasm)

Question 15

What are the characteristics of eosinophil?

Answer 15

1) Its nucleus is usually bilobed

2) Its cytoplasm contains large, coarse, reddish or orange granules

Question 16

What are the characteristics of basophil?

Answer 16

1) Its nucleus is usually indistinct and obscured by the granules
2) Its cytoplasm contains large purplish-black or dark blue granules

Question 17

What is the meaning of CU?

Answer 17

Conventional Unit

Question 18

What is the meaning of SI?

Answer 18

International System of Units

Question 19

What is the NV of neutrophil segmenter in CU?

Answer 19

50 - 70%

Question 20

What is the NV of lymphocyte in CU?

Answer 20

18 - 42%

Question 21

What is the NV of neutrophilic band in CU?

Answer 21

0 - 5%

Question 22

What is the NV of monocyte in CU?

Answer 22

2 - 11%

Question 23

What is the NV of eosinophil in CU?

Answer 23

1 - 3%

Question 24

What is the NV of basophil in CU?

Answer 24

0 - 2%

Question 25

What is the NV of neutrophil segmenter in SI?

Answer 25

0.50 - 0.70

Question 26

What is the NV of lymphocyte in SI?

Answer 26

0.18 - 0.42

Question 27

What is the NV of neutrophilic band in SI?

Answer 27

0 - 0.5

Question 28

What is the NV of monocyte in SI?

Answer 28

0.2 - 0.11

Question 29

What is the NV of eosinophil in SI?

Answer 29

0.01 - 0.03%

Question 30

What is the NV of basophil in SI?

Answer 30

0 - 0.02%

Question 31

What are the different types of stains used in diff WBC counting?

Answer 31

1) Romanowsky stains

2) Panoptic stains

Question 32

What are the components of Romanowsky stains?

Answer 32

1) Methylene blue (or its oxidative product: azure B)

2) Eosin B or eosin Y

Question 33

What is the purpose of the dyes present in Romanowsky stains?

Answer 33

The dyes produce multiple colors when used on cells and cellular components

Question 34

Why are the stains (or Romanowsky stains) present in Romanowsky stains are considered polychromatic?

Answer 34

Because these stains (or Romanowsky stains) has the capability to produce multiple colors when used on cells and cellular components

Question 35

What is Wright’s stain?

Answer 35

It is the most satisfactory in general routine hematologic studies

Question 36

What are the components of Wright’s stain?

Answer 36

1) Oxidized methylene blue

2) Eosin azures

Question 37

What is Giemsa stain?

Answer 37

It is an excellent stain for the demonstration of inclusion bodies and intracellular parasites as well as for staining WBCs

Question 38

What are the components of Giemsa stain?

Answer 38

1) Eosin Y w/ azure blue

2) Methylene blue in methanol w/ glycerin

Question 39

What is Leishman, Jenner, and May-Grunwald (stain)?

Answer 39

It is similar to Wright’s stain except for the method used to oxidize methylene blue

Question 40

What are panoptic stains?

Answer 40

These are stains that consists of Romanowsky stain and another dye

Question 41

What are the examples of Panoptic stains?

Answer 41

1) Wright’s-Giemsa
2) Jenner-Giemsa
3) May-Grunwald-Giemsa