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HEMA LAB > hemacytometer > Flashcards

hemacytometer Flashcards

1
Q

primarily used to count the cellular elements of the blood, including RBCs, WBCs, and platelets.

A

hemacytometer

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2
Q

the counting chamber used in the hemacytometer for manual counting is the __________

A

Improved Neubauer

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3
Q

elements in a hemacytometer kit

A

counting chamber, RBC pipette, WBC pipette, accessories (thick cover slip & suction device)

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4
Q

Odd One Out: Part of the hemacytometer kit

RBC pipette
Thick coverslip
Hemoglobin meter
WBC pipette

A

hgb meter

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5
Q

purpose of the central secondary square in the improved Neubauer counting chamber

A

count RBCs

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6
Q

Select All That Apply: Assist in blood cell counting in hemacytometer kit

Thick coverslip
RBC pipette
Centrifuge
Suction device
WBC pipette

A

Thick coverslip, RBC pipette, Suction device, WBC pipette

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7
Q

Select All That Apply: Types of counting chambers used in blood cell counting

Improved Neubauer
Speirs-Levy
Thoma
Fuchs-Rosenthal
Bass-Jones

A

all

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8
Q

T/F The Improved Neubauer counting chamber is divided into parallel platforms with the central platform being 0.1 mm lower than the lateral platforms.

A

T

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9
Q

The ruling of the central platform in the Improved Neubauer counting chamber contains __________ primary square(s) measuring ______ (9 square mm).

A

1 ; 3 x 3 mm

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10
Q

Select All That Apply: Central secondary square in Improved Neubauer chamber

1 Contains 25 tertiary squares
2 Used for counting WBCs
3 The tertiary squares measure 0.2 x 0.2 mm
4 5 of the 25 tertiary squares are used for RBC counting

A

1,3,4

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11
Q

T/F central platform of the Improved Neubauer chamber is separated by a moat that prevents overflow between the platforms.

A

true

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12
Q

Sequence: Manual RBC count using Improved Neubauer chamber

1 Count RBCs in 5 of the 25 tertiary squares in the central secondary square.
2 Place a thick coverslip over the chamber.
3 Fill the chamber with diluted blood.
4 View under a microscope using appropriate magnification.

A

2 3 4 1

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13
Q

OOO: 4 corner secondary squares of the Improved Neubauer chamber

1 16 tertiary squares
2 0.25 x 0.25 mm square size
3 Used for RBC counting
4 Used for WBC counting

A

3 used for RBC counting

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14
Q

depth of the central platform of the Improved Neubauer counting chamber

A

0.1 mm

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15
Q

Each of the four corner secondary squares of the Improved Neubauer chamber contains __________ tertiary squares measuring ____ mm x ___ mm , which are used for __________ counting.

A

16, 0.25 x 0.25 mm, WBC

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16
Q

T/F Laboratory automation has eliminated the need for manual counting using a hemacytometer.

A

F - manual is still used when automation fails / for crosschecking

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17
Q

The thickness of the __________ used with the hemacytometer is important to ensure accurate measurements during blood cell counting.

A

cover slip

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18
Q

The central platform of the Improved Neubauer counting chamber contains __________ parallel platforms extending across the slide and separated by moats.

A

three

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19
Q

central platform of the Improved Neubauer chamber is subdivided by what feature

A

transverse groove (moat)

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20
Q

T/F There are two primary ruled areas in one counting chamber

A

T

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21
Q

OOO: Area routinely used for cell counting

1 WBC counting in the four corners of the primary square
2 RBC counting in the center of the secondary square
3 WBC Differential Counting in peripheral blood films
4 Platelet counting in four corner secondary squares

A

4 - platelets are counted in the central secondary square

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22
Q

RBC counting is performed by counting cells in __________ corners and the center of tertiary squares in the central secondary square.

A

four

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23
Q

Select All That Apply: Area of cell counting in the hemacytometer

1 The area for WBC counting is 4 mm²
2 Platelets are counted in the whole central secondary square
3 The area of RBC counting is 5/25 of a tertiary square
4 The total magnification for WBC counting is 400x

A

1,2,3

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24
Q

T/F For RBC and platelet counting, we use a high power objective with a total magnification of 400x

A

t

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25
Q

Odd One Out: Lenss used in cell counting

1 Low power objective for WBC counting (10x)
2 Oil immersion objective for WBC differential counting (100x)
3 High power objective for RBC counting (40x)
4 Low power objective for platelet counting (20x)

A

4 - platelets are counted using the high power objective (40x)

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26
Q

For WBC differential counting, we use the __________ objective with a total magnification of __________

A

Oil immersion, 1000x

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27
Q

Select All That Apply: Steps for counting cells in a hemacytometer

1 Use the inverted L rule for counting cells
2 Count the cells that touch the top and the right
3 Use the total magnification of 1000x for RBC counting
4 Avoid duplicating cells by following a consistent method

A

1,4

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28
Q

Cell type counted using a low power objective (10x): WBC

A

WBC

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29
Q

T/F When counting cells in a hemacytometer, we count all cells in sight, including those which touch the four borders of the square

A

F - count cells touching the top and left borders and exclude those touching the bottom and right borders

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30
Q

counting area for platelets in the hemacytometer

A

whole central secondary square (1 mm²)

31
Q

T/F The major difference between the Neubauer and Improved Neubauer chambers lies in the number of tertiary squares in the central square.

A

T

32
Q

The Neubauer central secondary square has __________ tertiary squares, whereas the improved Neubauer has __________ tertiary squares.

A

16, 25

33
Q

size of each tertiary square in the Neubauer chamber

A

0.25 x 0.25 mm

34
Q

OOO: Difference between the Neubauer and Improved Neubauer chambers

1 Central square size
2 Number of tertiary squares
3 Overall depth of the chamber
4 Quality of RBC counting

A

3 - di nmn nagbagago depth oa k

35
Q

T/F Fuchs-Rosenthal counting chamber is primarily used for low cell counts such as eosinophil count and spinal fluid count

A

T

36
Q

The Fuchs-Rosenthal chamber has a grid area of __________ and a depth of __________

A

4 mm x 4 mm, 0.2 mm

37
Q

volume of one large square in the Fuchs-Rosenthal chamber

A

0.2 μL

38
Q

Select All That Apply: Fuchs-Rosenthal chamber

1 It has 16 smaller squares in the central square
2 The depth is 0.1 mm
3 The complete counting chamber has a volume of 3.2 μL
4 Used for leukopenia blood specimen counts

A

1 3 4

39
Q

T/F: In the Fuchs-Rosenthal counting chamber, to calculate the WBC count per μL, the total cell count is divided by 3.

A

T

40
Q

how many sections in Speirs-Levy counting chamber

A

4 sections ( 2 each side)

41
Q

Select All That Apply: Speirs-Levy counting chamber

1 It has 4 sections in total
2 Each section has 10 squares measuring 1 x 1 mm
3 It has a depth of 0.1 mm
4 commonly used for rbc counting

A

1, 2

*4 - pde nmn kaso most commonly sa bacteria nd other cells dw

42
Q

the Speirs-Levy chamber’s depth is __________; each section contains __________ squares

A

0.2 mm, 10

43
Q

Select All That Apply: Speirs-Levy counting chamber

1 4 sections in total
2 each section has 10 squares measuring 1 x 1 mm
3 depth of 0.1 mm
4 commonly used for RBC counting

A

1 2

44
Q

OOO: Speirs-Levy chamber

1 2 horizontal rows of squares
2 4 sections
3 Central secondary square with 25 smaller squares
4 Total area of 10 square mm

A

3

45
Q

The Speirs-Levy chamber has a total area of __________ square mm, while the Fuchs-Rosenthal chamber’s complete volume is __________ μL.

A

10, 3.2

46
Q

T/F Fuchs-Rosenthal and Speirs-Levy counting chambers are both designed for routine high cell counts like RBC counting.

A

F - designed for low cell counts (eosinophils, leukopenia)

47
Q

T/F: The Thoma pipette is primarily used for counting red blood cells (RBCs) and white blood cells (WBCs) in a blood sample.

A

T

48
Q

T/F The Thoma pipette is used for dilution before performing a cell count

A

T

49
Q

the thoma pipette consists of several parts including a bulb, stem, bore in the stem, and a ________ in the bulb

A

bead

50
Q

usual dilution for a WBC count using a Thoma pipette

A

1:20

51
Q

RBC pipette vs WBC pipette

Volume of bulb
Size of bore in stem
Calibration
Usual dilution
Color of bead

A

Volume of bulb (rbc - 100 units ; wbc 10 units)
Size of bore in stem (rbc -Smaller ; wbc - bigger)
Calibration (rbc - 0.5, 1.0, 101 ; wbc - 0.5, 1.0, 11)
Usual dilution (RBC - 1:200; WBC - 1:20)
Color of bead (RBC- red, WBC - white/colorless)

52
Q

T/F In WBC counting, it is necessary to lyse the RBCs because they would obstruct the counting process.

A

T

53
Q

For lysing RBCs in a WBC count, a ________ solution or a ________% acetic acid solution is used.

A

hypotonic, 3-5

54
Q

Select All That Apply: Thoma pipettes

1 The RBC pipette bulb volume is 100 units
2 The WBC pipette is used for immediate processing without lysing
3 The WBC pipette has a larger bore than the RBC pipette
4 The calibration for the RBC pipette includes 0.5, 1.0, and 101

A

1 3 4

55
Q

Select All That Apply: Thoma pipettes

1The RBC pipette has a smaller bore than the WBC pipette
2 The WBC pipette uses isotonic solution for dilution
3 The bulb of the RBC pipette holds 100 units
4 The WBC pipette is designed to lyse RBCs for counting

A

1 3 4

56
Q

volume of the bulb in a WBC Thoma pipette

A

10 units

57
Q

When aspirating blood into the Thoma pipette, it is important to mix it slightly until __________

A

the bulb is filled

58
Q

T/F In RBC counting with a Thoma pipette, there is no need for lysing the RBCs, and processing can be done immediately.

A

T

59
Q

T/F Thoma pipette is used for dilution before performing a cell count.

A

T

60
Q

T/F The stem of both the RBC and WBC Thoma pipettes is equivalent to 1 unit.

A

T

61
Q

The usual dilution for the RBC Thoma pipette is __________, while for the WBC Thoma pipette it is __________

A

1:200, 1:20

62
Q

T/F isotonic solution like NSS should be used in the RBC count to prevent lysis of red blood cells.

A

T

63
Q

The WBC pipette uses a hypotonic solution, such as __________, to lyse the RBCs.

A

acetic acid

64
Q

How long should the RBCs be lysed in the WBC count?

A

10 mins

65
Q

Select All That Apply: Thoma pipette

1 The RBC pipette can be used for WBC counting.
2 The WBC pipette can be used for RBC counting.
3 The dilution should be exact for accurate counting.
4 The Thoma pipette is not suitable for cell counts.

A

1 2 3

66
Q

T/F Disposable counting chambers such as Neubauer can use ordinary coverslips.

A

F - thick coverslips are needed

67
Q

The ordinary coverslip cannot be used because it has an _________ surface.

A

uneven

68
Q

purpose of omitting the first 5-6 drops when using the RBC pipette

A

remove diluent from the stem

69
Q

T/F The Thoma pipette can be used for both RBC and WBC counting, with specific dilutions depending on the situation.

A

T

70
Q

If blood is aspirated at the 0.5 mark of the RBC pipette, what is the dilution factor?`

A

1:200

70
Q

If you aspirate blood at the 0.1 mark of the WBC pipette, what is the dilution?

A

1:1000

71
Q

T/F When performing dilutions using the Thoma pipette, the stem is included in the dilution calculation.

A

F

72
Q

Select All That Apply:
Diluting fluids used to prevent rouleaux formation

A. Formal citrate
B. Gowers solution
C. Isotonic saline
D. Hypertonic solution

A

A B C

HEMA LAB (4 decks)

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