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HEMA LAB > [3S] reticulocyte count > Flashcards

[3S] reticulocyte count Flashcards

1
Q

What is the penultimate erythroid cell in the maturation sequence

A

reticulocyte

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2
Q

The young erythrocyte, formed when the nucleus of the late normoblasts is lost through extrusion, is called a ________.

A

Reticulocyte

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3
Q

t/f Reticulocytes are mature red blood cells that lack any residual RNA.

A

F- Reticulocytes contain residual RNA called “reticulum”

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4
Q

What type of material is found in reticulocytes and is visible with a supravital stain?

A

Residual RNA (reticulum)

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4
Q

Select: Which of the following dyes can be used to stain reticulocytes?
A) New methylene blue
B) Brilliant cresyl blue
C) Eosin
D) Wright’s stain

A

A B

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5
Q

What method is used to collect peripheral blood for reticulocyte count?

A

Pricking

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5
Q

removal of the nucleus in reticulocytes occurs through ________

A

Extrusion

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5
Q

OOO: characteristic of reticulocytes
A) Contains residual RNA
B) Nucleus is removed through extrusion
C) Ribosomal content increases as the cell matures
D) The nucleus is visible under supravital staining

A

D

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5
Q

t/f Reticulocyte count is used to monitor therapeutic measures for anemia

A

t

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6
Q

What hematologic test serves as an index of bone marrow activity and RBC production?

A

Reticulocyte count

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7
Q

Select: reticulocyte count
A) EDTA-anticoagulated whole blood
B) Serum
C) Peripheral blood
D) Heparinized plasma

A

A C

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8
Q

t/f Final results of a reticulocyte count from EDTA-anticoagulated blood are affected by storage temperature after 24 hours

A

F - Results are not affected even after 24 hours, irrespective of storage temperature.

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8
Q

What is the principle of the automated reticulocyte analyzer Sysmex R-1000?

A

Flow cytometry

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9
Q

t/f The dry method for reticulocyte counting uses a stain that gives a dull red color.

A

F - The stain, new methylene blue, gives a sharper blue color.

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10
Q

OOO: microscopic method

A) Rapid Method of Schilling
B) Cook, Meyer
C) Sysmex R-1000
D) Tureen

A

C

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10
Q

The Miller Disc Method uses an optical disc in the _______ of a microscope to facilitate reticulocyte counting.

A

Eyepiece or ocular

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10
Q

Select: Microscopic reticulocyte counting

A) Wet Method
B) Sysmex R-1000
C) Miller Disc Method
D) Dry Method

A

A C D

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11
Q

minimum number of cells that should be counted in the smaller square for the Miller Disc Method

A

112 cells

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12
Q

Computation (Miller Disc Method)
Reticulocytes counted in the large square: 16
RBCs counted in the small square: 115

A

ReticulocyteCount(%)=(NumberofReticulocytes(largesquare) / NumberofRBCs(smallsquare))×100

ReticulocyteCount(%)=(16/115)×100=13.91%

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12
Q

t/f According to the College of American Pathologists, a manual reticulocyte count should be based on at least 2000 RBCs.

A

f - at least 1000

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12
Q

T/F In the wet method, the reticulocyte count can be performed without covering the slide with a coverslip

A

F - mixture must be covered with a coverslip lined with Vaseline

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12
Q

Select: Components of the wet method procedure for reticulocyte counting

A) Place a drop of freshly filtered stain on a clean slide
B) Use a microscope equipped with a Miller disc
C) Cover the mixture with a clean coverslip lined with Vaseline
D) Mix blood and stain in equal volumes

A

A C D

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13
Q

Select: Reticulocyte microscopic methods

A) Brilliant cresyl blue
B) New methylene blue
C) Wright’s stain
D) Bromcresol green

A

B

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14
Q

most commonly employed manual technique for reticulocyte counting in clinical laboratories in the Philippines

A

dry method

14
Q

t/ In the Dry Method, reticulocyte smears are prepared immediately after mixing the blood and stain.

A

F - mixture is allowed to stand at room temperature for 15-30 minutes before preparing smears

14
Q

In the Dry Method, reticulocytes are counted in ______ successive fields of vision

A

10

14
Q

Select: Dry Method procedure

A) Mix blood and freshly filtered stain in equal volumes.
B) Allow the mixture to stand at 4°C.
C) Prepare smears and let them dry.
D) Count reticulocytes under Oil Immersion Objective (OIO).

A

A C D

14
Q

OOO: Dry Method
A) Freshly filtered stain
B) Coverslip lined with Vaseline
C) Glass slide
D) Oil Immersion Objective

A

B

14
Q

denominator used in the formula for calculating reticulocyte count in the Dry Method

A

1000

15
Q

t/f In the Dry Method, the formula for % Reticulocyte can be simplified to “Number of Reticulocytes / 10.”

A

T

15
Q

The factor 100 in the formula for reticulocyte count is used to express the result in _______.

A

percentage %

16
Q

Compute: Number of reticulocytes seen in 10 fields: 20. Reticulocyte count in percentage?

A

%ReticulocyteCount= (NumberofReticulocytes / 1000 )×100

(20/1000) ×100=2%

16
Q

Select: Reference ranges for reticulocyte count in babies

A) 0.5-1.5%
B) 2.0-6.0%
C) 5-15 x 10^-3
D) 20-60 x 10^-3

A

B D

16
Q

OOO: Adults

A) 0.5-1.5%
B) 5-15 x 10^-3
C) 2.0-6.0%
D) 0.5-2.5%

A

C

17
Q

Neonates have a higher reticulocyte count because they have more ________ in their bone marrow

A

Hematopoietic tissues

17
Q

T/F According to Rodak’s, the reticulocyte count in adults is 0.5-2.5%.

A

T

18
Q

Why do we need to correct the reticulocyte count in patients with low hematocrit levels?

A

Because the percentage of reticulocytes may be falsely elevated as the whole blood contains fewer RBCs

18
Q

The denominator in the formula for corrected reticulocyte count is ______ because it represents the average hematocrit percentage.

A

45

18
Q

A patient with a hematocrit of less than 25% should have a corrected reticulocyte count of 2% to 3% to compensate for moderate anemia.

A

F - should be 3% to 5%

18
Q

What does the absolute reticulocyte count measure?

A

actual number of reticulocytes in 1 liter or µL of blood

19
Q

The reference range for the absolute reticulocyte count is ______ to ______ x 10⁹/L.

A

20-115

19
Q

t/f The absolute reticulocyte count can also be reported as the number of cells per µL if the RBC count is expressed in conventional units.

A

t

19
Q

OOO: shift reticulocytes

A) Released prematurely from the bone marrow to compensate for anemia
B) Stay longer in peripheral blood circulation
C) Mature immediately upon entering circulation
D) Contribute to reticulocyte counts for more than one day

A

C

19
Q

When polychromasia is reported in RBC morphology, reticulocyte count is ______ because shifted reticulocytes contribute for more than one day.

A

falsely increased

20
Q

Select: Characteristics of automated techniques for measuring reticulocyte production

A) Uses Immature Reticulocyte Fraction (IRF)
B) Relies on the manual calculation of RPI
C) Assesses early bone marrow response
D) Requires mathematical adjustment

A

A C D

20
Q

An RPI greater than ______ indicates adequate bone marrow response, while an RPI less than ______ indicates inadequate response.

A

3 2

21
Q

Computation:
Corrected reticulocyte count: 2%
Patient’s hematocrit: 30%
Maturation time: 2 days

RPI= CorrectedReticulocyteCount / MaturationTime(days)
​
What is the RPI?

A

2/2 = 1 [INADEQUATE]

*RPI greater than 3 Adequate BM response
*RPI less than 2 Inadequate BM response

22
Q

OOO: Source of error in reticulocyte count
A) Incorrect proportion of blood and dye in anemic patients
B) Inadequate mixing before preparing the smear
C) Refractile bodies due to poor drying
D) Use of automated flow cytometry

A

D

22
Q

T/F Heinz bodies, Pappenheimer bodies, and Howell-Jolly bodies can be confused with reticulocytes.

A

T

23
Q

When using the Miller Disc method, it is important to follow the ______ rule to avoid bias.

A

Edge

23
Q

The maturation time of reticulocytes for a patient with a hematocrit of 35-39% is ______ days.

A

1.5

23
Q

t/f The maturation time for patients with a hematocrit of <15% is 3 days.

A

T

23
Q

Select: Hematocrit ranges with the shortest reticulocyte maturation time

A) 25-34%
B) 40-45%
C) <15%
D) 15-24%

A

B

23
Q

In the formula for calculating RPI, the patient’s hematocrit is used to determine the ______ time (in days).

A

maturation

23
Q

Select: Errors in reticulocyte counts
A) Incorrect proportion of blood and dye in anemic patients
B) Poor mixing of the blood-dye mixture before smear preparation
C) Use of a 1:1 ratio in all cases
D) Moisture during drying leading to refractile bodies

A

A B D

24
Q

T/F Reticulocytes should be mixed after 15–30 minutes of incubation time before preparing the smear because they have a higher specific gravity than other RBCs.

A

F - lower

25
Q

Moisture or poor drying can cause ______ bodies, which may be mistaken for reticulocytes.

A

refractile

25
Q

What adjustment should be made to the blood and dye proportion for anemic and polycythemic patients instead of the standard 1:1 ratio?

A

Adjust the proportion based on the hematocrit level to ensure accurate results.

HEMA LAB (4 decks)

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