Hem I and II/Hemostasis and Thrombosis Flashcards
What are the 4 general stages of hemostasis?
- Transient vasoconstriction
- Platelet adhesion to surface of disrupted vessel
- Platelet degranulation
- Platelet aggregation
When injury damages subendothelial tissue, what factor is released?
von Willebrand factor
Via which receptor does vWF contribute to platelet adhesion?
Gp1b to result in platelet adhesion
What two sources do vWF come from?
Weibel-Palade bodies of endothelial cells (vast majority) and some from alpha-granules of platelets
What is the disease which is a Gp1b deficiency? What is the result?
Bernard-Soulier disease; impaired platelet adhesion which leads to bleeding
What two factors are released by activated endothelial cells?
They synthesize tissue factor and secrete plasminogen activator inhibitor (PAI) which limits fibrinolysis
What are the following skin bleeding signs called:
1-2 mm
>3 mm
>1 cm
1-2 mm = petechiae (usually due to platelet deficiency or vit C deficiency; also sign of thrombocytopenia)
3-5 mm = purpura (also due to vasculitis or trauma)
>1 cm are ecchymoses (bruises)
What causes immune thrombocytopenic purpura (ITP)?
Autoimmune production of IgG against platelet antigens (e.g., GPIIb/IIIa)
In what organ does the events that eliminate platelets in immune thrombocytopenic purpura take place?
Most of the events take place in the spleen; the spleen will make the plasma cells that create the antibodies and they have macrophages that consume the antibody bound platelets
What are the two types of cytoplasmic granules that platelets have, and what do each contain?
Alpha granules: fibrinogen, factor V and VIII, vWF
Delta granules: ADP, ATP, calcium, serotonin, epinephrine
What would the PT/PTT values be for ITP?
Normal PT/PTT; it is a disorder of primary hemostasis and the coagulation cascade is unaffected.
What is microangiopathic hemolytic anemia?
Is the pathologic formation of platelet microthrombi in small vessels; the thrombi formation consumes platelets and shears RBCs resulting in hemolytic anemia with schistocytes
What two diseases is microangiopathic hemolytic anemia seen?
thrombocytopenic purpura and hemolytic uremic syndrome
What is the genetic cause of TTP?
Decreased ADAMTS13 which normally cleaves vWF Thus the resulting large multimers of vWF lead to platelet adhesion.
What is the cause of hemolytic uremic syndrome (HUS)?
Due to endothelial damage by drugs or via infection (esp. with undercooked beef)
What is the PT/PTT value for microangiopathic hemolytic anemia diseases?
Both are normal because this is a disease of primary hemostasis, not of coagulation cascade
What is the genetic defect in Glanzmann thromasthenia?
Due to a genetic GPIIb/IIIa deficiency; impairs platelet aggregation