heat denaturation experiment Flashcards
1
Q
what is the enzyme source
A
yeast
2
Q
what is the substrate and constant ph
A
hydrogen peroxide / buffer PH 9
3
Q
procedure of experiment
A
place yeast in test tube and boil at 100 C
add heated yeast and buffer solution to graduated cylinder
add hydrogen peroxide
stand cylinder until 25 C temperature is reached
repeat steps using unheated sample
4
Q
results of experiment
A
unheated enzyme = foam reduced
heated enzyme = no foam produced
5
Q
what is optimum activity
A
working at maximum rate
6
Q
what is a denudrated enzyme
A
loss of enzyme / function
