enzyme immobilization and test for alcohol by yeast Flashcards
1
Q
what is meant by immobilized
A
fixed to each other
2
Q
advantages of immobilized cells
A
can be re-used
3
Q
name of compound from which beads are formed
A
alginate
4
Q
materials used in enzyme immobilization
A
yeast / sodium alginate / calcium chloride / sucrose
5
Q
what is the procedure / explain enzyme immobilization
A
mix sodium alginate, water and yeast in beaker
release mixture into calcium chloride one drop at a time
beads will form
6
Q
result of immobilized enzymes
A
free yeast has presence of glucose after 0 minutes and after 2 minutes
immobilized yeast has NO presence of yeast after 0 minutes and has presence after 2 minutes
free yeast is cloudy / Immobilised yeast is is clear
