Haemocytometer cell counting

Question 0

% of dead cells

Answer 0

= (dead/total) x 100%

Question 1

What is the point of using this technique?

Answer 1

• calculate cell concentration
• count on left and top lines
• blue means dead cells with trypan blue dye

Question 2

% viable cells

Answer 2

=(alive/total) x100%

Question 3

of cells per ML of culture

Answer 3

Total/4
0.1 ul x 10,000 = 1ml

of cells in 1 square x 10,000= cells per ml

*** pay attention to ul amount. Multiple if necessary at the end

Question 4

Trypan blue method

Answer 4

• used to determine viable and non viable cells
• blue stained means cell isn’t viable
• see how effective dye is on cell culture