HACEK Flashcards
all species of haemophilus require special one o two growth factors
X and V factors
haemophilus X factor is called
heat-stable factor
X factor is a heat-stable
hemin
V factor is heat-labile
nicotinamide adenine dinucleotide (NAD)
Medium commonly used to isolate haemophilus spp
chocolate agar incubated 5% CO2
Agent of pink eye conjunctivitis
Haemphilus influezae
Infection cause by H.influenzae biotype Aegyptus that has 70% mortality rate
Brazilian purperic fever
Small coccobacilli or short rods with occasionally cells appearing as tangled filaments
Haemphilus haemolyticus
It produce filamentous forms hemin and NAD
H.parainfluenzae
Described as “schools of fish”,Buboes
H.ducreyi
Haemophilus spp. Grow on SBA very close to colonies of bacteria that can produce V factor.this phenomenon is called
Satelliting
A selective medium for isolation of H.influenzae
Horse blood bacitracin agar
H.ducreyi requires special media to grow what are those?
Mueller hinton-based chocolate agar
Heart infusion-based agar
Direct detection methods
*gram stain
*Haemophilus spp. Stain a pale pink
*Acridine orange stain
Antigen detection (H.influenzae)
*particle agglutination assays
*Latex test
Molecular methods
*PCR
*16s ribosomal ribonucleic acid
*Pulsedfield gel electrophoresis (GOLD STANDARD)
Cultures for H.ducreyi should be incubated at?
33°C
Haemophilus quad plate
Q1:BHI agar w/ hemin
Q2:BHI agar w/ isovitale
Q3:BHI agar w/ Xand V factor
Q4:horse blood agar with V factor
An alternative method for differentiating the heme-producing species of haemophilus
Porphyrin test
Bordetella primary isolation does require a special culture medium
Potato glycerol blood
B.pertussis os beat isolated on
Reagan lowe agar
The colonies of B.pertussis on reagan lowe agar
Smooth,convex,and pearl like(MERCURY DROPS)
