HACEK

Question 1

all species of haemophilus require special one o two growth factors

Answer 1

X and V factors

Question 2

haemophilus X factor is called

Answer 2

heat-stable factor

Question 3

X factor is a heat-stable

Answer 3

hemin

Question 4

V factor is heat-labile

Answer 4

nicotinamide adenine dinucleotide (NAD)

Question 5

Medium commonly used to isolate haemophilus spp

Answer 5

chocolate agar incubated 5% CO2

Question 6

Agent of pink eye conjunctivitis

Answer 6

Haemphilus influezae

Question 7

Infection cause by H.influenzae biotype Aegyptus that has 70% mortality rate

Answer 7

Brazilian purperic fever

Question 8

Small coccobacilli or short rods with occasionally cells appearing as tangled filaments

Answer 8

Haemphilus haemolyticus

Question 9

It produce filamentous forms hemin and NAD

Answer 9

H.parainfluenzae

Question 10

Described as “schools of fish”,Buboes

Answer 10

H.ducreyi

Question 11

Haemophilus spp. Grow on SBA very close to colonies of bacteria that can produce V factor.this phenomenon is called

Answer 11

Satelliting

Question 12

A selective medium for isolation of H.influenzae

Answer 12

Horse blood bacitracin agar

Question 13

H.ducreyi requires special media to grow what are those?

Answer 13

Mueller hinton-based chocolate agar
Heart infusion-based agar

Question 14

Direct detection methods

Answer 14

*gram stain
*Haemophilus spp. Stain a pale pink
*Acridine orange stain

Question 15

Antigen detection (H.influenzae)

Answer 15

*particle agglutination assays
*Latex test

Question 16

Molecular methods

Answer 16

*PCR
*16s ribosomal ribonucleic acid
*Pulsedfield gel electrophoresis (GOLD STANDARD)

Question 17

Cultures for H.ducreyi should be incubated at?

Answer 17

33°C

Question 18

Haemophilus quad plate

Answer 18

Q1:BHI agar w/ hemin
Q2:BHI agar w/ isovitale
Q3:BHI agar w/ Xand V factor
Q4:horse blood agar with V factor

Question 19

An alternative method for differentiating the heme-producing species of haemophilus

Answer 19

Porphyrin test

Question 20

Bordetella primary isolation does require a special culture medium

Answer 20

Potato glycerol blood

Question 21

B.pertussis os beat isolated on

Answer 21

Reagan lowe agar

Question 22

The colonies of B.pertussis on reagan lowe agar

Answer 22

Smooth,convex,and pearl like(MERCURY DROPS)