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BACTERIOLOGY > Aerobes Gram-positive Bacilli > Flashcards

Aerobes Gram-positive Bacilli Flashcards

1
Q

Club shape or coryneform

A

Corynebacterium

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2
Q

Cells are arranged in “Palisades” or to form V “Chinese letter “appearance

A

Corynebacterium

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3
Q

“Diptheria like”

A

Diphtheroid

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4
Q

Disease caused by C.diphtheriae

A

Diphtheria

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5
Q

Majoy virulence factor associated with C.diphtheria

A

Diphtheria toxin

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6
Q

Bacteria that is highly pleomorphic and appears to have V and L formations

A

C.diphtheria

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7
Q

The metachromatic areas of C.diphtheria which stain more intensely than other parts are called?

A

Babes-Ernts granules

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8
Q

C.diphtheriae usually obtained on a medium containing blood or serum such as?

A

Loeffler serum or Pai agars

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9
Q

The modified agar from Tinsdale medium

A

Cystine-tellurite blood agar (CTBA)

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10
Q

Characteristics of C.diphtheriae in Tinsdale agar

A

Brown-black colonies with gray brown halo

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11
Q

Stimulates the growth of C.diphtheriae

A

Loeffler medium

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12
Q

Colony morphology of C.diphtheriae in Loeffler medium

A

Gray to white, translucent colonies

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13
Q

What are the only 3 Corynebacterium that forms brown halo?

A

C.diphthriae,C.ulcerans,C.pseudotubercolosis

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14
Q

What test that distinguishes C.diphtheriae to other corynebacterium

A

Urease test (-)

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15
Q

The in vitro diphtheria toxin detection was first described by?

A

Elek

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16
Q

associated with prosthetic joint infection and has been reported to cause bloodstream
infection and endocarditis

A

Corynebacterium amycolatum

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17
Q

most common cause of Corynebacterium-associated prosthetic valve endocarditis in adults.

A

Corynebacterium jeikeium

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18
Q

isolated from skin ulcers and exudative pharyngitis.

A

Corynebacterium ulcerans

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19
Q

mastitis in cattle and other domestic and wil animals

A

Corynebacterium ulcerans

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20
Q

A corynebacterium most commonly
associated with UTIs.

A

Corynebacterium urealyticum

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21
Q

What medium do C.urealyticum grows better

A

Lipid contaning medium

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22
Q

The most reliable test to identify toxigenic strains of Corynebacterium spp.

A

MALDI-TOF MS

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23
Q

Molecular methods to identify C. diphtheriae

A

ribotyping, pulsed-field gel electrophoresis, and multilocus sequence typing

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24
Q

colonize human fetus

A

Listeria monocytogenes

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25
Q

Virulence factor of Listeria monocytogenes

A

Listeriolysin O

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26
Q

L.monocytogenes grows at what Celsius?

A

4°C

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27
Q

monocytogenes grows at 4° C, a technique called?

A

Cold enrichment

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28
Q

Medium for Listeria monocytogenes

A

SBA and chocolate agar, nutrient agars, brain-heart infusion
broth and thioglycolate broth

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29
Q

What Listeria that has a Narrow zone of beta hemolysis on sheep blood agar

A

Listeria monocytogenes

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30
Q

In wet mount preparations, L. monocytogenes exhibits?

A

Tumbling motility

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31
Q

L. monocytogenes In motility medium shows _____ pattern

A

Umbrella

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32
Q

L. monocytogenes produces a ________ type hemolysis with
the CAMP test.

A

Block

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33
Q

What antibodies can be detected in cases of
listeriosis

A

Antilisteriolysin O IgG

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34
Q

normal human oropharyngeal microbiota/endocarditis

A

Rothia dentocariosa

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35
Q

A bacteria that consist of several serovars (commonly associated with human infection include serovar 1&2)

A

Erysipelothrix rhusiopathiae

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36
Q

A skin infection that is painful and spread lesions on hands and fingers

A

Erysipeloid

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37
Q

A bacteria that grows on a gelatin stab culture that yields a “test tube brush-like pattern” at 22°C

A

E.rhusiopathiae

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38
Q

Mediums for E.rhusiopathiae

A

5% SBA and Choc agar, CNA agar

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39
Q

Associated with bacterial vaginosis in humans

A

Gardnerella vaginalis

40
Q

Large, squamous epithelial cells with numerous attached small rods

A

Clue cells

41
Q

The medium of choice for G vaginalis

A

Human blood bilayer tween (HBT) agar,CNA agar

42
Q

A bacteria that does not grow on blood culture broth with SPS

A

G.vaginalis

43
Q

What odor does G.vaginalis smell using the whiff test with 10% KOH

A

Fishy odor

44
Q

Immunocompromised lactobacillus spp.

A

Bacteremia

45
Q

Organisms enters the bloodstream while chewing brushing teat or dental procedure resulting in?

A

Bacteremia and endocarditis

46
Q

What agar does lactobacillus spp. grow?

A

Tomato juice agar

47
Q

The only bacteria that has been recovered from clinical specimens

A

A.haemolyticum

48
Q

What are the two trueperella spp. That when infections occur they generally are cutaneous

A

T.pyogenes and T.bernardie

49
Q

Has a morphology with a beaded appearance

A

Nocardia

50
Q

It is a chronic, localized,painless, subcutaneous infection

A

Mycetoma

51
Q

It is the predominant cause of these skin infections (skin abscess or cellulitis)

A

N.brasiliensis

52
Q

It has been considered the most common isolated pathogen

A

N.asteroides

53
Q

They are etiologic agents of mycetomas

A

Actinomadura madurae and actinomadura pelletieri

54
Q

Exudative dermatitis with scab formation (dermatophilosis)

A

Dermatophilus congolensis

55
Q

Actinomycetoma and other skin infection

A

Nocardiopsis dassonvillei

56
Q

It is established as an human pathogen associated with actinomycotic mycetoma

A

Streptomyces somaliensis

57
Q

They grow with mycelia forms that fragment into a so called “Nocardioform”

A

Gordonia

58
Q

On SBA this bacteria has a morphology resembling klebsiella and form a salmonpink pigment

A

Rhodococcus

59
Q

The identification of this bacteria is the salmon pink pigment and diptheroid gram positive rods with traces of branching

A

Rhodococcus

60
Q

Bacteria catheter associated sepsis

A

Dietzia spp

61
Q

It is the agent of “Whipples diseases”

A

Tropheryma whipplei

62
Q

The presences of characteristics periodic __________ is strongly suggested of Whipples disease

A

Acid-schiff staining

63
Q

If gram staining,branching or partially branching is observed what stain should be performed?

A

Modified acid fast stain

64
Q

Gram positive BEADED,BRANCHING FILAMENTS can be confirmed as?

A

Nocardia

65
Q

A histologic stain that can also detect the presence of of actinomycetes

A

Gomoris methenamine-silver stain(GMS)

66
Q

Non-acid fast aerobic actinomycetes can grow on what agar?

A

SBA,CHOC,Sabouraud-dextrose (SDA),Lowenstein-jensen,brain-heat infusion agar

67
Q

Nocardiae require a minimum of how many hrs of incubation?

A

48-72 hrs

68
Q

Selective media of Nocardia spp.

A

Modified Thayer-martin

69
Q

Nocardia spp. can grow on non selective (BYCE agar)

A

Buffered charcoal yeast extract agar

70
Q

Colonies of Nocardia spp.

A

Chalky,matte,powdery appearance,similar to breadcrumbs

71
Q

Facultive anaerobic bacilli that form endospores

A

Bacillus

72
Q

What Bacillus that can cause WOOLSORTERS DISEASE

A

Bacillus anthracis

73
Q

Virulance factor of B.anthracis

A

Capsule exotoxins

74
Q

Capsule exotoxins of B.anthracis

A

Edema toxin and lethal toxin

75
Q

Anthrax toxin consist of three proteins

A

Protective agent(PA),Edema factor (EF),Lethal factor (LF)

76
Q

What anthrax toxin that serve as binding molecule

A

Protective agent (PA)

77
Q

Progressive stages from an erythematous papule to ulceration and to black scars

A

Cuteneous anthrax

78
Q

Also know as WOOLSORTERS DISEASE AND RAGPICKERS DISEASE

A

Pulmonary anthrax

79
Q

Cuteneous anthrax specimen should be collected from underneath the____?

A

Eschar

80
Q

Bacillus anthracis histochemecal testing should be placed in a?

A

10% formalin

81
Q

In Inhalation anthrax specimen physician may collect ______ or ______?

A

Biopsy of brochial or pleural tissue

82
Q

It can be used for the growth of endospores from clinical specimen, effective for killing vegetative cells and regain spores

A

Heat shock treatment

83
Q

B.anthracis on gram staining shows the appearance of?

A

Bamboo rods

84
Q

What color of B.anthracis vegetative cells on gram stain

A

Red

85
Q

What color of the B.anthracis spores in stain

A

Green

86
Q

B.anthracis mediums

A

Phenylethyl alcohol agar (PEA)
PLET agar
SBA 5%

87
Q

Is has theappearance of “Comet tail or medusa head” on SBA

A

B.anthracis

88
Q

The colonies of this bacteria has been described a having the appearance or characteristics of a BEATEN EGG WHITES

A

B.anthracis

89
Q

After incubation of B.anthracis for 3-6 hrs at 37°C is shows large, spherical bacilli in chains,this phenomenon is called?

A

String of pearls

90
Q

Food poisoning caused by B.cereus takes two forms

A

Diarrheal and emetic

91
Q

3 toxin implicated in the diarrheal symptoms

A

Hemolysin BL,Non hemolytic enterotoxin,cytotoxin K

92
Q

B.cereus diarrheal syndrome incubation period

A

8-16 hrs

93
Q

This form of B.cereus is associated with nausea and vomiting to 1-5 hrs after ingestion of contaminated food

A

Emetic form

94
Q

What are the mediums for B.cereus

A

Mannitol,egg yolk and polymyxin B agar (MEYP or MYP)
PEMBA agar
B.cereus medium (BCM)

95
Q

Serelogic methods for detection of B.cereus toxin in food and feces

A

The Oxoid BCET-RPLA
TECRA VIA

BACTERIOLOGY (5 decks)

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