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BACTERIOLOGY > Aerobes Gram-positive Bacilli > Flashcards

Aerobes Gram-positive Bacilli Flashcards

1
Q

Club shape or coryneform

A

Corynebacterium

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2
Q

Cells are arranged in “Palisades” or to form V “Chinese letter “appearance

A

Corynebacterium

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3
Q

“Diptheria like”

A

Diphtheroid

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4
Q

Disease caused by C.diphtheriae

A

Diphtheria

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5
Q

Majoy virulence factor associated with C.diphtheria

A

Diphtheria toxin

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6
Q

Bacteria that is highly pleomorphic and appears to have V and L formations

A

C.diphtheria

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7
Q

The metachromatic areas of C.diphtheria which stain more intensely than other parts are called?

A

Babes-Ernts granules

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8
Q

C.diphtheriae usually obtained on a medium containing blood or serum such as?

A

Loeffler serum or Pai agars

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9
Q

The modified agar from Tinsdale medium

A

Cystine-tellurite blood agar (CTBA)

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10
Q

Characteristics of C.diphtheriae in Tinsdale agar

A

Brown-black colonies with gray brown halo

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11
Q

Stimulates the growth of C.diphtheriae

A

Loeffler medium

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12
Q

Colony morphology of C.diphtheriae in Loeffler medium

A

Gray to white, translucent colonies

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13
Q

What are the only 3 Corynebacterium that forms brown halo?

A

C.diphthriae,C.ulcerans,C.pseudotubercolosis

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14
Q

What test that distinguishes C.diphtheriae to other corynebacterium

A

Urease test (-)

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15
Q

The in vitro diphtheria toxin detection was first described by?

A

Elek

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16
Q

associated with prosthetic joint infection and has been reported to cause bloodstream
infection and endocarditis

A

Corynebacterium amycolatum

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17
Q

most common cause of Corynebacterium-associated prosthetic valve endocarditis in adults.

A

Corynebacterium jeikeium

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18
Q

isolated from skin ulcers and exudative pharyngitis.

A

Corynebacterium ulcerans

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19
Q

mastitis in cattle and other domestic and wil animals

A

Corynebacterium ulcerans

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20
Q

A corynebacterium most commonly
associated with UTIs.

A

Corynebacterium urealyticum

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21
Q

What medium do C.urealyticum grows better

A

Lipid contaning medium

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22
Q

The most reliable test to identify toxigenic strains of Corynebacterium spp.

A

MALDI-TOF MS

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23
Q

Molecular methods to identify C. diphtheriae

A

ribotyping, pulsed-field gel electrophoresis, and multilocus sequence typing

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24
Q

colonize human fetus

A

Listeria monocytogenes

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25
Virulence factor of Listeria monocytogenes
Listeriolysin O
26
L.monocytogenes grows at what Celsius?
4°C
27
monocytogenes grows at 4° C, a technique called?
Cold enrichment
28
Medium for Listeria monocytogenes
SBA and chocolate agar, nutrient agars, brain-heart infusion broth and thioglycolate broth
29
What Listeria that has a Narrow zone of beta hemolysis on sheep blood agar
Listeria monocytogenes
30
In wet mount preparations, L. monocytogenes exhibits?
Tumbling motility
31
L. monocytogenes In motility medium shows _____ pattern
Umbrella
32
L. monocytogenes produces a ________ type hemolysis with the CAMP test.
Block
33
What antibodies can be detected in cases of listeriosis
Antilisteriolysin O IgG
34
normal human oropharyngeal microbiota/endocarditis
Rothia dentocariosa
35
A bacteria that consist of several serovars (commonly associated with human infection include serovar 1&2)
Erysipelothrix rhusiopathiae
36
A skin infection that is painful and spread lesions on hands and fingers
Erysipeloid
37
A bacteria that grows on a gelatin stab culture that yields a "test tube brush-like pattern" at 22°C
E.rhusiopathiae
38
Mediums for E.rhusiopathiae
5% SBA and Choc agar, CNA agar
39
Associated with bacterial vaginosis in humans
Gardnerella vaginalis
40
Large, squamous epithelial cells with numerous attached small rods
Clue cells
41
The medium of choice for G vaginalis
Human blood bilayer tween (HBT) agar,CNA agar
42
A bacteria that does not grow on blood culture broth with SPS
G.vaginalis
43
What odor does G.vaginalis smell using the whiff test with 10% KOH
Fishy odor
44
Immunocompromised lactobacillus spp.
Bacteremia
45
Organisms enters the bloodstream while chewing brushing teat or dental procedure resulting in?
Bacteremia and endocarditis
46
What agar does lactobacillus spp. grow?
Tomato juice agar
47
The only bacteria that has been recovered from clinical specimens
A.haemolyticum
48
What are the two trueperella spp. That when infections occur they generally are cutaneous
T.pyogenes and T.bernardie
49
Has a morphology with a beaded appearance
Nocardia
50
It is a chronic, localized,painless, subcutaneous infection
Mycetoma
51
It is the predominant cause of these skin infections (skin abscess or cellulitis)
N.brasiliensis
52
It has been considered the most common isolated pathogen
N.asteroides
53
They are etiologic agents of mycetomas
Actinomadura madurae and actinomadura pelletieri
54
Exudative dermatitis with scab formation (dermatophilosis)
Dermatophilus congolensis
55
Actinomycetoma and other skin infection
Nocardiopsis dassonvillei
56
It is established as an human pathogen associated with actinomycotic mycetoma
Streptomyces somaliensis
57
They grow with mycelia forms that fragment into a so called "Nocardioform"
Gordonia
58
On SBA this bacteria has a morphology resembling klebsiella and form a salmonpink pigment
Rhodococcus
59
The identification of this bacteria is the salmon pink pigment and diptheroid gram positive rods with traces of branching
Rhodococcus
60
Bacteria catheter associated sepsis
Dietzia spp
61
It is the agent of "Whipples diseases"
Tropheryma whipplei
62
The presences of characteristics periodic __________ is strongly suggested of Whipples disease
Acid-schiff staining
63
If gram staining,branching or partially branching is observed what stain should be performed?
Modified acid fast stain
64
Gram positive BEADED,BRANCHING FILAMENTS can be confirmed as?
Nocardia
65
A histologic stain that can also detect the presence of of actinomycetes
Gomoris methenamine-silver stain(GMS)
66
Non-acid fast aerobic actinomycetes can grow on what agar?
SBA,CHOC,Sabouraud-dextrose (SDA),Lowenstein-jensen,brain-heat infusion agar
67
Nocardiae require a minimum of how many hrs of incubation?
48-72 hrs
68
Selective media of Nocardia spp.
Modified Thayer-martin
69
Nocardia spp. can grow on non selective (BYCE agar)
Buffered charcoal yeast extract agar
70
Colonies of Nocardia spp.
Chalky,matte,powdery appearance,similar to breadcrumbs
71
Facultive anaerobic bacilli that form endospores
Bacillus
72
What Bacillus that can cause WOOLSORTERS DISEASE
Bacillus anthracis
73
Virulance factor of B.anthracis
Capsule exotoxins
74
Capsule exotoxins of B.anthracis
Edema toxin and lethal toxin
75
Anthrax toxin consist of three proteins
Protective agent(PA),Edema factor (EF),Lethal factor (LF)
76
What anthrax toxin that serve as binding molecule
Protective agent (PA)
77
Progressive stages from an erythematous papule to ulceration and to black scars
Cuteneous anthrax
78
Also know as WOOLSORTERS DISEASE AND RAGPICKERS DISEASE
Pulmonary anthrax
79
Cuteneous anthrax specimen should be collected from underneath the____?
Eschar
80
Bacillus anthracis histochemecal testing should be placed in a?
10% formalin
81
In Inhalation anthrax specimen physician may collect ______ or ______?
Biopsy of brochial or pleural tissue
82
It can be used for the growth of endospores from clinical specimen, effective for killing vegetative cells and regain spores
Heat shock treatment
83
B.anthracis on gram staining shows the appearance of?
Bamboo rods
84
What color of B.anthracis vegetative cells on gram stain
Red
85
What color of the B.anthracis spores in stain
Green
86
B.anthracis mediums
Phenylethyl alcohol agar (PEA) PLET agar SBA 5%
87
Is has theappearance of "Comet tail or medusa head" on SBA
B.anthracis
88
The colonies of this bacteria has been described a having the appearance or characteristics of a BEATEN EGG WHITES
B.anthracis
89
After incubation of B.anthracis for 3-6 hrs at 37°C is shows large, spherical bacilli in chains,this phenomenon is called?
String of pearls
90
Food poisoning caused by B.cereus takes two forms
Diarrheal and emetic
91
3 toxin implicated in the diarrheal symptoms
Hemolysin BL,Non hemolytic enterotoxin,cytotoxin K
92
B.cereus diarrheal syndrome incubation period
8-16 hrs
93
This form of B.cereus is associated with nausea and vomiting to 1-5 hrs after ingestion of contaminated food
Emetic form
94
What are the mediums for B.cereus
Mannitol,egg yolk and polymyxin B agar (MEYP or MYP) PEMBA agar B.cereus medium (BCM)
95
Serelogic methods for detection of B.cereus toxin in food and feces
The Oxoid BCET-RPLA TECRA VIA

BACTERIOLOGY (5 decks)

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