Growing Bacteria Flashcards
1
Q
What is the first step for growing bacteria?
A
Sterilise agar jelly
2
Q
What is the second step of growing bacteria?
A
Sterilise the inoculating loop
3
Q
Why do we sterilise the agar jelly and inoculating loop?
A
To get rid of any unwanted bacteria
4
Q
Why do we leave the loop to cool after heating it on the blue flame?
A
Prevent killing bacteria sample
5
Q
Once the loop has cooled what do we do?
A
Dip the loop into the bacteria sample and transfer it into the agar jelly
6
Q
Once the bacteria sample is in the agar jelly what do we do?
A
Gently rub the loop onto the agar jelly to isolate a single colony of bacteria
7
Q
Why do we tape the lid shut?
A
To prevent bacteria from the air contaminating the agar
8
Q
Why do we incubate the agar jelly at 25 degrees celcius?
A
Prevents growth of pathogens
