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Science Flashcards > Gram staining > Flashcards

Gram staining Flashcards

1
Q

Thick peptidoglycan cell wall

Gram + or - ?

A

Gram +

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2
Q

Gram +

A
  • thick peptidoglucan layer
  • no outere membrain layer
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3
Q

No outer Membrain

Gram + or - ?

A

Gram +

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4
Q

Penicilin provents cross linking chains from forming

Gram + or - ?

A

Gram +

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5
Q

Penicilin inhibits enzyme from working

Gram + or - ?

A

Gram +

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6
Q

Cell wall synthisis is disrupted

affected by antibiotics

Gram + or - ?

A

Gram +

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7
Q

New cells grow abnormaly

affected by antibiotics

Gram + or - ?

A

Gram +

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8
Q

Cells burst due to weakened cell wall - Lysis

affected by antibiotics

Gram + or - ?

A

Gram +

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9
Q

Thin peptidoglycan cell wall

Gram + or - ?

A

Gram -

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10
Q

Peptidoglycan surrounded by a thin outer membrain layer

Gram + or - ?

A

Gram -

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11
Q

Gram -

A
  • thin peptidoglycan layer
  • thin outer membrane
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12
Q

Antibiotic must cross cell wall to be effective

Gram + or - ?

A

Gram -

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13
Q

Ridgid cell wall to prevent exess water access

Gram + or - ?

A

Gram -

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14
Q

Cells dont burst

are not affected by antibiotics

Gram + or - ?

A

Gram -

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15
Q

Cells continue to grow

are not affected by antibiotics

Gram + or - ?

A

Gram -

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16
Q

Stains purple

Gram + or - ?

A

Gram +

17
Q

Stains pink

Gram + or - ?

A

Gram -

18
Q

Wich stain dosn’t effect gram - bacteria?

A

Crystal Violet

19
Q

Why is Safranin able to stain Gram - but not Gram+?

A

Safranin is smaller than crystal violet and can penitrate the outer membrain layer of gram - to reach the peptidoglycan layer underneeth BUT in gram +, the darker crystal violet stain is too dark for the pink to be seen, but it dose still stain the gram +

20
Q

How dose penicilin work on gram +?

A
  • Penicilin disrupts the cell wall synthisis
  • Wich stops an enzyme, that forms cross bridges, from working
  • This weakens the short protines they are made of
  • Leading to the cells being unable to hold up under osmosis and bursting (lysis)
21
Q

Why dosn’t penicilin work on gram -?

A

Gram - has an outer cell memebrain that acts as a barier that protects the peptidoglycan from the penicilin.
- this keeps the cell wall strong/ridgid
- prevents the cell from bursting under osmosis

22
Q

Gram staining technique

  • Add b——- cultures to microscope slide
  • Add C——- V—– stain and rince with lugiol’s iodeen and ethanol
  • Add s——- stain and rince with water
  • Allow to — and add cover slip before viewing under microscope
A
  • Add bacterial cultures to microscope slide
  • Add crystal violet stain and rince with lugiol’s iodeen and ethanol
  • Add safranin stain and rince with water
  • Allow to dry and add cover slip before viewing under microscope

Science Flashcards (7 decks)

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