Gram Positive Rods Flashcards
Aerobic Actinomycetes
Branch & break off to look like coccobacillus
Capnophilic - likes CO2
Acid fast - mycolic acid in cell walls
Kinyoun stain - weak decolorizer causing partial acid fast to fuschia color
Actinomycetes
Acts like a fungus
Bacteria - prokaryote
Fungus - eukaryote
Branchers
Nocardia species
N. asteroides
N. brasiliensis
N. asteroides
Rarely a pathogen Infection - pulmonary & cutaneous Draining sinuses - pathway for something to drain out Sulfur granules - bacteria in granules Resistant to lysozyme Substrates & sequencing -casein agar - milk agar -clearing around it to use up = hydrolysis
N. brasiliensis
Uses casein & tyrosine
Gordona/Gordonia
Opportunistic pathogen
Actinomadura
brancher
heaped colony
Streptomyces
brancher
not partially acid fast
not resistant to lysozyme
Bacillus
Spore forming
Spores/endospores
withstand heat & chemicals
gram stain - spores don’t pick up stain
schaffer-fulton stain stains spores with malachite green
Anthrax types
Cutaneous
Inhalation
Gastrointestinal
Cutaneous anthrax
Eschar - black lesion
Gastrointestinal anthrax
identifies B. anthracis
nonmotile
non hemolytic
Penicillin disk test for anthrax
20mm+ = sensitive
zone of inhibition
string of pearls induced by presence of penicillin
Sodium bicarbonate
media that helps bacteria form capsule