Gram Positive Rods Flashcards
Aerobic Actinomycetes
Branch & break off to look like coccobacillus
Capnophilic - likes CO2
Acid fast - mycolic acid in cell walls
Kinyoun stain - weak decolorizer causing partial acid fast to fuschia color
Actinomycetes
Acts like a fungus
Bacteria - prokaryote
Fungus - eukaryote
Branchers
Nocardia species
N. asteroides
N. brasiliensis
N. asteroides
Rarely a pathogen Infection - pulmonary & cutaneous Draining sinuses - pathway for something to drain out Sulfur granules - bacteria in granules Resistant to lysozyme Substrates & sequencing -casein agar - milk agar -clearing around it to use up = hydrolysis
N. brasiliensis
Uses casein & tyrosine
Gordona/Gordonia
Opportunistic pathogen
Actinomadura
brancher
heaped colony
Streptomyces
brancher
not partially acid fast
not resistant to lysozyme
Bacillus
Spore forming
Spores/endospores
withstand heat & chemicals
gram stain - spores don’t pick up stain
schaffer-fulton stain stains spores with malachite green
Anthrax types
Cutaneous
Inhalation
Gastrointestinal
Cutaneous anthrax
Eschar - black lesion
Gastrointestinal anthrax
identifies B. anthracis
nonmotile
non hemolytic
Penicillin disk test for anthrax
20mm+ = sensitive
zone of inhibition
string of pearls induced by presence of penicillin
Sodium bicarbonate
media that helps bacteria form capsule
India ink stain
helps identify capsule
B. cereus
causes food poisoning
caused by grains - cereal, rice
emetic - vomiting
PEA phenyl ethyl agar
selective
B. anthracis
hemolysis - motility - hydrolysis - PEA - penicillin - lecithinase production + catalase +
B. cereus
hemolysis + motility + hydrolysis + PEA + penicillin + lecithinase production + catalase +
