Gram positive cocci Flashcards
Colono morphology of Staphylococcus in Sheep Blood Agar( SDA)
Smooth, yellow, white or off-white with a diameter of 1-2mm
True or False: Staphylococcus may exhibit B hemolysis
True
Differential media contains chromagenic substrates that yield a certain colony color
Chromagar
A quick test that differentiate off white or gray colonies of Staphyloccocus from Streptococcus
Catalase test(most common)
two quick tests that differentiate Staphylococcus from Micrococcus
Modified Oxidase and Lysostaphin
A quick tests for both bound and free coagulase?
Coagulase Test
Latex Agglutination Test for Staph
Slidex Staph Bacti Staph Staphytect Staphylase Staph Latex Slide Test Staphtex Set-RPL Latex Staph
Kits that utilize passive hemagglutination for S. aureus?
Staphyslide
HemaStaph
StaphyloSlide
S. auteus can further be indentified by its ability to produce
DNAse
Mannitol Salt Agar
(ferment mannitol)
Conventional Method: coagulase negative Staph tests
Carbohydrate utilization (SXTFMML)
Urease and Nitrate Reduction
Phosphate
(CUP)
Conventional Method: S.saphrophyticus which cause UTI can be identified by..
Resistance to Novobiocin
Conventional method: that differentiate Staphylococcus from Micrococcus
Bacitracin (suscep M.)
Acid Production from glucose(+ S)
Identifying Strips: AP ID 32 Staph and API staph use for identifying
Coagulase negative staphylococci.
Colony morphology of Micrococcus
Dull, White colonies that may produce Tan, Pink or Orange color with 1-2 mm diameter. Larger gram-positive cocci arranged in Tetrads.
Quick tests: in helping differentiate Micrococcus from Staphyloccoci
Catalase test
Modified Oxidase test
Conventional Method: to separate Micrococci from Staph
Glucose fermentation Lysostaphin Furazolidone Bacitracin (Good Luck Fuck Boy)
Colonoy Morphology of Streptococcus
smaller on Sheep Blood Agar with 1mm of diameter
Two agars that enhance growth of Streptococci
Columbia Naladixic Acid Agar
Phenylethyl Alcohol Agar
(CNAA and PEAA)
Separation of Streptococcusis hemolysis
Alpha - hemolysis
Alpha - prime hemolysis
Beta - hemolysis
Gamma - hemolysis
Quick test: for differentiating Staph from Streph especially from gamma hemolytic strep
Catalase test
Quick tests: that provide definitive identification of strep
Latex Agglutination
Coagglutination test
(LC)
Quick tests: to help aid identification of Enterococci
Pyrrolidonyl Arylamidase
Leucine Aminopeptidase
(PYR and LAP tests)
Quick test: rapic identification of enterococci?
Remel disks
Quic tests: rapid tests for S.pneumoniae
Dryspot Pneumo
Pneumoslide
(DP)