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SEM -Bacteriology > Gram positive cocci > Flashcards

Gram positive cocci Flashcards

1
Q

Colono morphology of Staphylococcus in Sheep Blood Agar( SDA)

A

Smooth, yellow, white or off-white with a diameter of 1-2mm

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2
Q

True or False: Staphylococcus may exhibit B hemolysis

A

True

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3
Q

Differential media contains chromagenic substrates that yield a certain colony color

A

Chromagar

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4
Q

A quick test that differentiate off white or gray colonies of Staphyloccocus from Streptococcus

A

Catalase test(most common)

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5
Q

two quick tests that differentiate Staphylococcus from Micrococcus

A

Modified Oxidase and Lysostaphin

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6
Q

A quick tests for both bound and free coagulase?

A

Coagulase Test

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7
Q

Latex Agglutination Test for Staph

A 
Slidex Staph
Bacti Staph 
Staphytect
Staphylase
Staph Latex Slide Test
Staphtex
Set-RPL Latex Staph
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8
Q

Kits that utilize passive hemagglutination for S. aureus?

A

Staphyslide
HemaStaph
StaphyloSlide

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9
Q

S. auteus can further be indentified by its ability to produce

A

DNAse
Mannitol Salt Agar
(ferment mannitol)

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10
Q

Conventional Method: coagulase negative Staph tests

A

Carbohydrate utilization (SXTFMML)
Urease and Nitrate Reduction
Phosphate

(CUP)

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11
Q

Conventional Method: S.saphrophyticus which cause UTI can be identified by..

A

Resistance to Novobiocin

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12
Q

Conventional method: that differentiate Staphylococcus from Micrococcus

A

Bacitracin (suscep M.)

Acid Production from glucose(+ S)

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13
Q

Identifying Strips: AP ID 32 Staph and API staph use for identifying

A

Coagulase negative staphylococci.

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14
Q

Colony morphology of Micrococcus

A

Dull, White colonies that may produce Tan, Pink or Orange color with 1-2 mm diameter. Larger gram-positive cocci arranged in Tetrads.

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15
Q

Quick tests: in helping differentiate Micrococcus from Staphyloccoci

A

Catalase test

Modified Oxidase test

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16
Q

Conventional Method: to separate Micrococci from Staph

A 
Glucose fermentation
Lysostaphin
Furazolidone
Bacitracin
(Good Luck Fuck Boy)
17
Q

Colonoy Morphology of Streptococcus

A

smaller on Sheep Blood Agar with 1mm of diameter

18
Q

Two agars that enhance growth of Streptococci

A

Columbia Naladixic Acid Agar
Phenylethyl Alcohol Agar
(CNAA and PEAA)

19
Q

Separation of Streptococcusis hemolysis

A

Alpha - hemolysis
Alpha - prime hemolysis
Beta - hemolysis
Gamma - hemolysis

20
Q

Quick test: for differentiating Staph from Streph especially from gamma hemolytic strep

A

Catalase test

21
Q

Quick tests: that provide definitive identification of strep

A

Latex Agglutination
Coagglutination test
(LC)

22
Q

Quick tests: to help aid identification of Enterococci

A

Pyrrolidonyl Arylamidase
Leucine Aminopeptidase
(PYR and LAP tests)

23
Q

Quick test: rapic identification of enterococci?

A

Remel disks

24
Q

Quic tests: rapid tests for S.pneumoniae

A

Dryspot Pneumo
Pneumoslide
(DP)

25
Q

Conventional Method for B hemolytic Strep

A 
Bacitracin disk
Suscpetibility to SXT
Camp Test
Hippurate Hydrolysis
Vogues Proskauer
Pyrrolidonyl Arylamidase
(Bull Shit HC VP)

*SXT - sulfonamide trimethoprim-sulfamethoxazole

26
Q

Conventional Test: Alpha Hemolytic

A

6% NaCl

Arginine hydrolysis
Acid from Mannitol

Optochin disk
Bile Solubility
Esculin Hydrolysis

27
Q

Conventional Tests: Enterococci

A

6.5% NaCl

Bile Esculin
SXT susceptibility

Motility
Vogues Proskauer
Pigment production
(6 Bull Shit MVP)

28
Q

Quick test: for identification of Aerococcus

A

Pyrrolidonyl Arylamidase

Leukocyte Alkaline Phosphatase

29
Q

Conventional Test: For Aerococcus

A

6.5% NaCl

Acid production from SorLMTre
Hippurate Hyrdolysis
Bile Esculin
6 Ahb like “abs”

30
Q

What are the Miscellenous Gram-Positive cocci?

A 
Alloiococcus
- from sputum, blood cultutes and tympanocentesis fluid
Rothia (Stomatococcus)
- from blood cultures
Globicatella
- from blood cultures
Helococcus
- from wounds
Vagococcus
-primarily zoonotic

SEM -Bacteriology (4 decks)

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